論文 - 詳細
| RRC ID | 77531 |
|---|---|
| 著者 | Jerard C, Madhusudanan P, Swamy A, Ravikumar K, Shankarappa SA. |
| タイトル | Secretome mediated interactions between sensory neurons and breast cancer cells. |
| ジャーナル | Int J Cancer |
| Abstract |
The role of the nervous system in aiding cancer progression and metastasis is an important aspect of cancer pathogenesis. Interaction between cancer cells and neurons in an in vitro platform is a simple and robust method to further understand this phenomenon. In our study, we aimed to examine in vitro reciprocal effect between breast cancer cells and cancer-sensitized peripheral primary sensory neurons. Secretome obtained from either cultured DRG neurons from tumor-burdened rats, or MRMT1 breast cancer cells were used to study neuronal and cancer cell reciprocity. We utilized neurite analysis, modified cell migration assay and cell signaling pathway inhibitors to determine neurite growth patterns and cell migration in PC12/DRG neurons and MRMT1 cells, respectively. MRMT1 secretome was found to induce significant neurite outgrowth in PC12 and primary sensory neurons. Secretome-induced neurite growth in PC12 cells was partly mediated by PI3K and ERK pathways, but not by adenylyl cyclase. Conversely, secretome from tumor-sensitized sensory neuron cultures induced increased rate of migration in cultured MRMT1 cells. Results from our study provide additional support to the hypothesis that both breast cancer cells and nerve terminals secrete signaling messengers that have a reciprocal effect on each other. |
| 巻・号 | 153(2) |
| ページ | 427-436 |
| 公開日 | 2023-7-15 |
| DOI | 10.1002/ijc.34529 |
| PMID | 37067100 |
| MeSH | Animals Cells, Cultured Ganglia, Spinal Neoplasms* / metabolism Neurites / metabolism PC12 Cells Rats Secretome* Sensory Receptor Cells Signal Transduction |
| IF | 5.145 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 27 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MRMT-1(RCB2860) |