論文 - 詳細
| RRC ID | 77606 |
|---|---|
| 著者 | Nishimura T, Kaneko S, Kawana-Tachikawa A, Tajima Y, Goto H, Zhu D, Nakayama-Hosoya K, Iriguchi S, Uemura Y, Shimizu T, Takayama N, Yamada D, Nishimura K, Ohtaka M, Watanabe N, Takahashi S, Iwamoto A, Koseki H, Nakanishi M, Eto K, Nakauchi H. |
| タイトル | Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation. |
| ジャーナル | Cell Stem Cell |
| Abstract |
Adoptive immunotherapy with functional T cells is potentially an effective therapeutic strategy for combating many types of cancer and viral infection. However, exhaustion of antigen-specific T cells represents a major challenge to this type of approach. In an effort to overcome this problem, we reprogrammed clonally expanded antigen-specific CD8(+) T cells from an HIV-1-infected patient to pluripotency. The T cell-derived induced pluripotent stem cells were then redifferentiated into CD8(+) T cells that had a high proliferative capacity and elongated telomeres. These "rejuvenated" cells possessed antigen-specific killing activity and exhibited T cell receptor gene-rearrangement patterns identical to those of the original T cell clone from the patient. We also found that this method can be effective for generating specific T cells for other pathology-associated antigens. Thus, this type of approach may have broad applications in the field of adoptive immunotherapy. |
| 巻・号 | 12(1) |
| ページ | 114-26 |
| 公開日 | 2013-1-3 |
| DOI | 10.1016/j.stem.2012.11.002 |
| PII | S1934-5909(12)00636-4 |
| PMID | 23290140 |
| MeSH | CD8-Positive T-Lymphocytes / cytology CD8-Positive T-Lymphocytes / metabolism Cell Differentiation / genetics Cell Differentiation / physiology* Cells, Cultured Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism Models, Biological T-Lymphocytes / cytology* T-Lymphocytes / metabolism |
| IF | 20.86 |
| リソース情報 | |
| ヒト・動物細胞 | OP9/G-DLL1(RCB2925) OP9/N-DLL1(RCB2927) |