| RRC ID |
77743
|
| 著者 |
Kawai K, Sasaki T, Saijo-Kurita K, Akaza H, Koiso K, Ohno T.
|
| タイトル |
Additive effects of antitumor drugs and lymphokine-activated killer cell cytotoxic activity in tumor cell killing determined by lactate-dehydrogenase-release assay.
|
| ジャーナル |
Cancer Immunol Immunother
|
| Abstract |
The effect of pretreatment with antitumor drugs on lymphokine-activated killer (LAK) cell cytotoxic activity, determined by lactate-dehydrogenase(LDH)-release assay, was investigated. LAK cells were induced by incubating peripheral blood lymphocytes of healthy donors in medium containing interleukin-2 (IL-2) and monoclonal anti-CD3 antibody for 6-7 days. A human lung squamous carcinoma cell line, SQ-5, was used as an adherent target. After 24 h exposure of the target cells to cisplatin, doxorubicin, or mitomycin C, the drugs were washed off and LAK cells were added at an E/T ratio of 5. During further incubation for 48 h, LDH release from cisplatin- or doxorubicin-pretreated target cells was markedly higher than that from non-pretreated target cells. The combination of cisplatin and LAK cells has an additive cytotoxic effect and that of mitomycin C and LAK cells does not; there may also be an additive effect late in the toxicity mechanism between doxorubicin and LAK cells.
|
| 巻・号 |
35(4)
|
| ページ |
225-9
|
| 公開日 |
1992-1-1
|
| DOI |
10.1007/BF01789327
|
| PMID |
1511457
|
| MeSH |
Antineoplastic Agents / pharmacology*
Carcinoma, Squamous Cell / enzymology
Carcinoma, Squamous Cell / pathology
Carcinoma, Squamous Cell / therapy
Cell Death / drug effects
Cell Survival / drug effects
Clinical Enzyme Tests / methods
Combined Modality Therapy
Cytotoxicity, Immunologic
Drug Synergism
Humans
Immunotherapy, Adoptive*
Killer Cells, Lymphokine-Activated / drug effects
Killer Cells, Lymphokine-Activated / immunology*
L-Lactate Dehydrogenase / analysis
L-Lactate Dehydrogenase / metabolism*
Lung Neoplasms / enzymology
Lung Neoplasms / pathology
Lung Neoplasms / therapy
Time Factors
Tumor Cells, Cultured / enzymology
|
| IF |
5.442
|
| リソース情報 |
| ヒト・動物細胞 |
SQ-5(RCB0110) |
