| RRC ID |
77816
|
| 著者 |
Ochiai Y, Suzuki C, Segawa K, Uchiyama Y, Nagata S.
|
| タイトル |
Inefficient development of syncytiotrophoblasts in the Atp11a-deficient mouse placenta.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
The P4-ATPases ATP11A and ATP11C function as flippases at the plasma membrane to translocate phosphatidylserine from the outer to the inner leaflet. We herein demonstrated that Atp11a-deficient mouse embryos died at approximately E14.5 with thin-walled heart ventricles. However, the cardiomyocyte- or epiblast-specific Atp11a deletion did not affect mouse development or mortality. ATP11C may have compensated for the function of ATP11A in most of the cell types in the embryo. On the other hand, Atp11a, but not Atp11c, was expressed in the mouse placenta, and the Atp11a-null mutation caused poor development of the labyrinthine layer with an increased number of TUNEL-positive foci. Immunohistochemistry and electron microscopy revealed a disorganized labyrinthine layer with unfused trophoblasts in the Atp11a-null placenta. Human placenta-derived choriocarcinoma BeWo cells expressed the ATP11A and ATP11C genes. A lack of ATP11A and ATP11C eliminated the ability of BeWo cells to flip phosphatidylserine and fuse when treated with forskolin. These results indicate that flippases at the plasma membrane play an important role in the formation of syncytiotrophoblasts in placental development.
|
| 巻・号 |
119(18)
|
| ページ |
e2200582119
|
| 公開日 |
2022-5-3
|
| DOI |
10.1073/pnas.2200582119
|
| PMID |
35476530
|
| PMC |
PMC9170144
|
| MeSH |
ATP Binding Cassette Transporter 1
Adenosine Triphosphatases / metabolism
Animals
Cell Membrane / metabolism
Female
Mice
Phosphatidylserines / metabolism
Placenta* / metabolism
Pregnancy
Trophoblasts* / metabolism
|
| IF |
9.412
|
| リソース情報 |
| ヒト・動物細胞 |
BeWo(RCB3678) |
