| RRC ID |
77827
|
| Author |
Kimura S, Naganuma S, Susuki D, Hirono Y, Yamaguchi A, Fujieda S, Sano K, Itoh H.
|
| Title |
Expression of microRNAs in squamous cell carcinoma of human head and neck and the esophagus: miR-205 and miR-21 are specific markers for HNSCC and ESCC.
|
| Journal |
Oncol Rep
|
| Abstract |
MicroRNAs (miRNAs) are non-coding small RNAs that regulate cell proliferation and functions by interfering with the translation of target mRNAs. Altered expression of miRNA is known to induce various human malignancies. We examined the expression of miRNAs in squamous cell carcinoma of human head and neck (HNSCC) and esophagus (ESCC), compared to that in normal squamous epithelia as well as malignancies of other organs. Microarray analysis showed up-regulation of miR-21, miR-16 and miR-30a-5p in HNSCC and ESCC cell lines compared to normal squamous epithelial cell lines, and consistent high expression of miR-205 and let-7a in both normal and malignant squamous epithelial cell lines. Validation study using real-time quantitative RT-PCR in formalin-fixed paraffin-embedded cancer tissues and paired normal epithelia obtained by Laser-captured microdissection revealed that miR-205 showed highest expression in both malignant and benign squamous epithelia, although it was less expressed in cell lines and tissues other than squamous epithelia. MiR-21, which is an oncogenic miRNA in various malignancies, was also up-regulated in HNSCC and ESCC compared to paired normal squamous epithelia. These results suggest that miR-205 might be a specific marker miRNA of both normal and malignant squamous epithelia, while miR-21 might be a putative oncogenic miRNA in HNSCC and ESCC.
|
| Volume |
23(6)
|
| Pages |
1625-33
|
| Published |
2010-6-1
|
| DOI |
10.3892/or_00000804
|
| PMID |
20428818
|
| MeSH |
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor / genetics*
Biomarkers, Tumor / metabolism
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / pathology
Esophageal Neoplasms / genetics*
Esophageal Neoplasms / pathology
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Head and Neck Neoplasms / genetics*
Head and Neck Neoplasms / pathology
Humans
Male
MicroRNAs / genetics*
Middle Aged
Oligonucleotide Array Sequence Analysis
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
|
| IF |
3.417
|
| Resource |
| Human and Animal Cells |
HSC-4(RCB1902)
HSC-3(RCB1975)
SAS(RCB1974)
LoVo(RCB1639)
HL60(RCB0041)
MOLT-4(RCB0206)
Hep G2
K562 |
