| RRC ID |
77829
|
| 著者 |
Uchida K, Akita Y, Matsuo K, Fujiwara S, Nakagawa A, Kazaoka Y, Hachiya H, Naganawa Y, Oh-Iwa I, Ohura K, Saga S, Kawai T, Matsumoto Y, Shimozato K, Kozaki K.
|
| タイトル |
Identification of specific autoantigens in Sjögren's syndrome by SEREX.
|
| ジャーナル |
Immunology
|
| Abstract |
We carried out SEREX (serological analysis of antigens by recombinant cDNA expression cloning) using sera from patients with Sjögren's syndrome (SjS) and investigated the frequencies of autoantibodies against autoantigens identified by SEREX in the sera of healthy individuals (HI) and patients with SjS, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). IFI16 and two kelch-like proteins, KLHL12 and KLHL7, were found to be novel autoantigens in SjS by SEREX. A markedly high frequency of anti-IFI16 autoantibodies was observed in the sera of SjS (SjS, 70%; RA, 13%; SLE, 33%; HI, 0%). Interestingly, all serum samples from SjS demonstrated immunoreactivity against one or both of IFI16 and SS-B/La. The presence of autoantibodies against KLHL12 and KLHL7 in the sera was significantly specific to SjS (23% and 17%, respectively), as they were not detected in RA, SLE or HI. Furthermore, we confirmed that transcripts of these autoantigens were expressed preferentially in the salivary glands and immuno-privileged testes. Our results suggest these autoantigens may be useful as serological markers for the clinical diagnosis of SjS and may play a crucial role as organ-specific autoantigens in the aetiopathogenesis of SjS. This study warranted clinical evaluations of autoantibodies against IFI16, KLHL12 and KLHL7 in combination with anti-SS-B/La autoantibodies.
|
| 巻・号 |
116(1)
|
| ページ |
53-63
|
| 公開日 |
2005-9-1
|
| DOI |
10.1111/j.1365-2567.2005.02197.x
|
| PII |
IMM2197
|
| PMID |
16108817
|
| PMC |
PMC1802401
|
| MeSH |
Adolescent
Adult
Aged
Aged, 80 and over
Autoantibodies / blood
Autoantigens / immunology
Autoantigens / metabolism*
Autoimmune Diseases / immunology*
Blotting, Northern
Blotting, Western
Female
Gene Library
Humans
Male
Microfilament Proteins / immunology
Microfilament Proteins / metabolism
Middle Aged
Nerve Tissue Proteins / immunology
Nerve Tissue Proteins / metabolism
Nuclear Proteins / immunology
Nuclear Proteins / metabolism
Phosphoproteins / immunology
Phosphoproteins / metabolism
Salivary Glands / immunology
Sjogren's Syndrome / immunology*
|
| IF |
5.016
|
| リソース情報 |
| ヒト・動物細胞 |
HSQ-89(RCB0789) |
