論文 - 詳細
| RRC ID | 77889 |
|---|---|
| 著者 | Ueno M, Nakata M, Kaneko Y, Iwami M, Takayanagi-Kiya S, Kiya T. |
| タイトル | fruitless is sex-differentially spliced and is important for the courtship behavior and development of silkmoth Bombyx mori. |
| ジャーナル | Insect Biochem Mol Biol |
| Abstract |
Sexual dimorphisms of the brain play essential roles in successful reproduction. Silkmoth Bombyx mori exhibits extensive sexual differences in sexual behavior, as well as their morphology. Although the neural circuits that transmit information about sex pheromone in the male brain are extensively analyzed, the molecular mechanisms that regulate their development are still elusive. In the present study, we focused on the silkmoth ortholog of fruitless (fru) as a candidate gene that regulates sexual dimorphisms of the brain. fru transcripts were expressed from multiple promoters in various tissues, and brain-specific transcripts were sex-specifically spliced, in a manner similar to Drosophila. Interestingly, fru was highly expressed in the adult female brain and the male larval testis. Analysis of CRISPR/Cas9-mediated fru knockout strains revealed that fru plays important roles in survival during late larval and pupal stages, testis development, and adult sexual behavior. fru mutant males exhibited highly reduced levels of courtship and low copulation rate, indicating that fru plays significant roles in the sexual behavior of silkmoths, although it is not absolutely necessary for copulation. In the fru mutant males, sexually dimorphic pattern of the odorant receptor expression was impaired, possibly causing the defects in courtship behavior. These results provide important clues to elucidate the development of sexual dimorphisms of silkmoth brains, as well as the evolution of fruitless gene in insects. |
| 巻・号 | 159 |
| ページ | 103989 |
| 公開日 | 2023-8-1 |
| DOI | 10.1016/j.ibmb.2023.103989 |
| PII | S0965-1748(23)00083-8 |
| PMID | 37453662 |
| MeSH | Animals Bombyx* / genetics Bombyx* / metabolism Courtship Drosophila / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / metabolism Female Male Nerve Tissue Proteins / genetics Sexual Behavior, Animal / physiology Transcription Factors / genetics |
| IF | 3.827 |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | p55 |