RRC ID 78057
Author Karitani Y, Yamada R, Matsumoto T, Ogino H.
Title UV mutagenesis improves growth potential of green algae in a green algae-yeast co-culture system.
Journal Arch Microbiol
Abstract It is known that co-cultivation of green algae with heterotrophic microorganisms, such as yeast, improves green algae's growth potential and carbon dioxide fixation, even under low CO2 concentration conditions such as the atmosphere. Introducing mutations into green algae is also expected to enhance their growth potential. In this study, we sought to improve the growth potential of a co-culture system of the green algae Chlamydomonas reinhardtii and the yeast Saccharomyces cerevisiae by introducing mutations into the green algae. Additionally, we performed a transcriptome analysis of the co-culture of the green algae mutant strain with yeast, discussing the interaction between the green algae mutant strain and the yeast. When the green algae mutant strain was co-cultured with yeast, the number of green algae cells reached 152 × 105 cells/mL after 7 days of culture. This count was 2.6 times higher than when the wild-type green algae strain was cultured alone and 1.6 times higher than when the wild-type green algae strain and yeast were co-cultured. The transcriptome analysis also indicated that the primary reason for the increased growth potential of the green algae mutant strain was its enhanced photosynthetic activity and nitrogen utilization efficiency.
Volume 206(2)
Pages 61
Published 2024-1-12
DOI 10.1007/s00203-023-03796-2
PII 10.1007/s00203-023-03796-2
PMID 38216809
MeSH Carbon Dioxide Chlorophyta* / genetics Coculture Techniques Mutagenesis Photosynthesis Saccharomyces cerevisiae* / genetics
Algae NIES-2238