RRC ID 78083
Author Hamada T, Komiya S, Yano H, Zenmyo M, Hiraoka K, Inoue A, Morimatsu M.
Title Modulation of fas-mediated apoptosis in osteosarcoma cell lines.
Journal Int J Oncol
Abstract Fas/APO-1 (or CD95) is a 45-kDa cell surface glycoprotein that transduces cellular death signals for apoptosis upon cross-linking with the Fas ligand, which is experimentally replaced by anti-Fas antibodies. We examined the Fas expression at the protein and mRNA levels, and susceptibility to anti-Fas-mediated apoptosis, in 14 osteosarcoma cell lines. Ten of 14 ostesarcoma cell lines constitutively expressed detectable levels of Fas on the cell surface with positive cell rates ranging from 10.2 to 72.4%. Four other cell lines expressed Fas almost exclusively in the cytoplasm. An antibody against Fas was able to induce Fas-mediated cell death only in two cell lines with high levels of surface Fas. In the presence of the protein synthesis inhibitor cyclohexamide, antibody to Fas led to an induction of apoptosis in ten of the osteosarcoma cell lines antibody-concentration dependently. These data suggest that Fas is a potential protein related to apoptosis in osteosarcomas when the sensitizing activity of cyclohexamide on anti-Fas-mediated cytotoxicity was exerted.
Volume 15(6)
Pages 1125-31
Published 1999-12-1
DOI 10.3892/ijo.15.6.1125
PMID 10568818
MeSH Antibodies, Monoclonal / pharmacology Apoptosis* Cell Survival / drug effects Culture Media, Conditioned / chemistry Cycloheximide / pharmacology DNA, Complementary / genetics Gene Expression Regulation, Neoplastic Genetic Variation Humans Immunohistochemistry Osteosarcoma / genetics Osteosarcoma / pathology Osteosarcoma / physiopathology* Protein Isoforms / analysis Protein Isoforms / genetics Protein Isoforms / physiology Protein Synthesis Inhibitors / pharmacology RNA, Neoplasm / genetics Tumor Cells, Cultured fas Receptor / genetics fas Receptor / immunology fas Receptor / physiology*
IF 3.899
Human and Animal Cells OST(RCB0454)