論文 - 詳細
| RRC ID | 78319 |
|---|---|
| 著者 | Kimira Y, Sato T, Sakamoto M, Osawa Y, Mano H. |
| タイトル | Collagen-Derived Dipeptide Pro-Hyp Enhanced ATDC5 Chondrocyte Differentiation under Hypoxic Conditions. |
| ジャーナル | Molecules |
| Abstract |
Chondrocytes are surrounded by a lower oxygen environment than other well-vascularized tissues with higher oxygenation levels. Prolyl-hydroxyproline (Pro-Hyp), one of the final collagen-derived peptides, has been previously reported to be involved in the early stages of chondrocyte differentiation. However, whether Pro-Hyp can alter chondrocyte differentiation under physiological hypoxic conditions is still unclear. This study aimed to investigate whether Pro-Hyp affects the differentiation of ATDC5 chondrogenic cells under hypoxic conditions. The addition of Pro-Hyp resulted in an approximately 18-fold increase in the glycosaminoglycan staining area compared to the control group under hypoxic conditions. Moreover, Pro-Hyp treatment significantly upregulated the expression of SOX9, Col2a1, Aggrecan, and MMP13 in chondrocytes cultured under hypoxic conditions. These results demonstrate that Pro-Hyp strongly promotes the early differentiation of chondrocytes under physiological hypoxic conditions. Therefore, Pro-Hyp, a bioactive peptide produced during collagen metabolism, may function as a remodeling factor or extracellular matrix remodeling signal that regulates chondrocyte differentiation in hypoxic cartilage. |
| 巻・号 | 28(12) |
| 公開日 | 2023-6-9 |
| DOI | 10.3390/molecules28124664 |
| PII | molecules28124664 |
| PMID | 37375217 |
| PMC | PMC10305454 |
| MeSH | Cell Differentiation / physiology Cells, Cultured Chondrocytes* / metabolism Collagen* / metabolism Dipeptides / metabolism Dipeptides / pharmacology Peptides / metabolism Peptides / pharmacology |
| IF | 3.267 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Youtube |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |