| RRC ID |
78439
|
| 著者 |
Lázaro J, Costanzo M, Sanaki-Matsumiya M, Girardot C, Hayashi M, Hayashi K, Diecke S, Hildebrandt TB, Lazzari G, Wu J, Petkov S, Behr R, Trivedi V, Matsuda M, Ebisuya M.
|
| タイトル |
A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals.
|
| ジャーナル |
Cell Stem Cell
|
| Abstract |
Differential speeds in biochemical reactions have been proposed to be responsible for the differences in developmental tempo between mice and humans. However, the underlying mechanism controlling the species-specific kinetics remains to be determined. Using in vitro differentiation of pluripotent stem cells, we recapitulated the segmentation clocks of diverse mammalian species varying in body weight and taxa: marmoset, rabbit, cattle, and rhinoceros. Together with mouse and human, the segmentation clock periods of the six species did not scale with the animal body weight, but with the embryogenesis length. The biochemical kinetics of the core clock gene HES7 displayed clear scaling with the species-specific segmentation clock period. However, the cellular metabolic rates did not show an evident correlation. Instead, genes involving biochemical reactions showed an expression pattern that scales with the segmentation clock period. Altogether, our stem cell zoo uncovered general scaling laws governing species-specific developmental tempo.
|
| 巻・号 |
30(7)
|
| ページ |
938-949.e7
|
| 公開日 |
2023-7-6
|
| DOI |
10.1016/j.stem.2023.05.014
|
| PII |
S1934-5909(23)00210-2
|
| PMID |
37343565
|
| PMC |
PMC10321541
|
| MeSH |
Animals
Basic Helix-Loop-Helix Transcription Factors* / metabolism
Biological Clocks
Cattle
Cell Differentiation
Gene Expression Regulation, Developmental
Humans
Mammals / metabolism
Mice
Pluripotent Stem Cells*
Rabbits
|
| リソース情報 |
| ヒト・動物細胞 |
EpiSC 129Ba1(AES0204)
rdES2-1(AES0174)
201B7(HPS0063) |
