論文 - 詳細
| RRC ID | 78489 |
|---|---|
| 著者 | Tojima T, Suda Y, Jin N, Kurokawa K, Nakano A. |
| タイトル | Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast. |
| ジャーナル | Elife |
| Abstract |
Cargo traffic through the Golgi apparatus is mediated by cisternal maturation, but it remains largely unclear how the cis-cisternae, the earliest Golgi sub-compartment, is generated and how the Golgi matures into the trans-Golgi network (TGN). Here, we use high-speed and high-resolution confocal microscopy to analyze the spatiotemporal dynamics of a diverse set of proteins that reside in and around the Golgi in budding yeast. We find many mobile punctate structures that harbor yeast counterparts of mammalian endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC) proteins, which we term 'yeast ERGIC'. It occasionally exhibits approach and contact behavior toward the ER exit sites and gradually matures into the cis-Golgi. Upon treatment with the Golgi-disrupting agent brefeldin A, the ERGIC proteins form larger aggregates corresponding to the Golgi entry core compartment in plants, while cis- and medial-Golgi proteins are absorbed into the ER. We further analyze the dynamics of several late Golgi proteins to better understand the Golgi-TGN transition. Together with our previous studies, we demonstrate a detailed spatiotemporal profile of the entire cisternal maturation process from the ERGIC to the Golgi and further to the TGN. |
| 巻・号 | 13 |
| 公開日 | 2024-3-19 |
| DOI | 10.7554/eLife.92900 |
| PII | 92900 |
| PMID | 38501165 |
| PMC | PMC10950332 |
| MeSH | Animals Endoplasmic Reticulum / metabolism Golgi Apparatus / metabolism Mammals Saccharomyces cerevisiae* / metabolism Saccharomycetales* trans-Golgi Network / metabolism |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 24 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |