RRC ID 78513
Author Ikeshima-Kataoka H, Skeath JB, Nabeshima Y, Doe CQ, Matsuzaki F.
Title Miranda directs Prospero to a daughter cell during Drosophila asymmetric divisions.
Journal Nature
Abstract Asymmetric cell division is a general process used in many developmental contexts to create two differently fated cells from a single progenitor cell. Intrinsic mechanisms like the asymmetric transmission of cell-fate determinants during cell division, and extrinsic cell-interaction mechanisms, can mediate asymmetric divisions. During embryonic development of the Drosophila central nervous system, neural stem cells called neuroblasts divide asymmetrically to produce another multipotent neuroblast and a ganglion mother cell (GMC) of more restricted developmental potential. Intrinsic mechanisms promote asymmetric division of neuroblasts: for example, the transcription factor Prospero localizes to the basal cell cortex of mitotic neuroblasts and then segregates exclusively into the GMC, which buds off from the basal side of the neuroblast. In the GMC, Prospero translocates to the nucleus, where it establishes differential gene expression between sibling cells. Here we report the identification of a gene, miranda, which encodes a new protein that co-localizes with Prospero in mitotic neuroblasts, tethers Prospero to the basal cortex of mitotic neuroblasts, directing Prospero into the GMC, and releases Prospero from the cell cortex within GMCs. miranda thus creates intrinsic differences between sibling cells by mediating the asymmetric segregation of a transcription factor into only one daughter cell during neural stem-cell division.
Volume 390(6660)
Pages 625-9
Published 1997-12-11
DOI 10.1038/37641
PMID 9403694
MeSH Alleles Amino Acid Sequence Animals Binding Sites Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Cycle Proteins / physiology* Cell Differentiation / genetics Cell Differentiation / physiology* Cell Division / physiology* Cloning, Molecular Drosophila / cytology Drosophila / embryology Drosophila / genetics Drosophila Proteins* Female Ganglia, Invertebrate / cytology Genes, Insect Male Molecular Sequence Data Mutagenesis Nerve Tissue Proteins / metabolism Nerve Tissue Proteins / physiology* Neurons / cytology* Nuclear Proteins / metabolism Nuclear Proteins / physiology* Protein Binding Stem Cells / cytology Stem Cells / metabolism Transcription Factors / metabolism Transcription Factors / physiology*
IF 42.779
Drosophila DGRC#119104 DGRC#119105 DGRC#119107 DGRC#119108 DGRC#119109 DGRC#119110