論文 - 詳細
| RRC ID | 78518 |
|---|---|
| 著者 | Yoshiura S, Ohta N, Matsuzaki F. |
| タイトル | Tre1 GPCR signaling orients stem cell divisions in the Drosophila central nervous system. |
| ジャーナル | Dev Cell |
| Abstract |
During development, directional cell division is a major mechanism for establishing the orientation of tissue growth. Drosophila neuroblasts undergo asymmetric divisions perpendicular to the overlying epithelium to produce descendant neurons on the opposite side, thereby orienting initial neural tissue growth. However, the mechanism remains elusive. We provide genetic evidence that extrinsic GPCR signaling determines the orientation of cortical polarity underlying asymmetric divisions of neuroblasts relative to the epithelium. The GPCR Tre1 activates the G protein oα subunit in neuroblasts by interacting with the epithelium to recruit Pins, which regulates spindle orientation. Because Pins associates with the Par-complex via Inscuteable, Tre1 consequently recruits the polarity complex to orthogonally orient the polarity axis to the epithelium. Given the universal role of the Par complex in cellular polarization, we propose that the GPCR-Pins system is a comprehensive mechanism controlling tissue polarity by orienting polarized stem cells and their divisions. |
| 巻・号 | 22(1) |
| ページ | 79-91 |
| 公開日 | 2012-1-17 |
| DOI | 10.1016/j.devcel.2011.10.027 |
| PII | S1534-5807(11)00479-5 |
| PMID | 22178499 |
| MeSH | Animals Blotting, Western Cell Division / physiology* Cell Polarity* Cells, Cultured Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism* Epithelial Cells / cytology* Epithelial Cells / metabolism GTP-Binding Protein alpha Subunits / genetics GTP-Binding Protein alpha Subunits / metabolism Immunoenzyme Techniques Immunoprecipitation NIMA-Interacting Peptidylprolyl Isomerase Neurons / cytology* Neurons / metabolism Peptidylprolyl Isomerase / genetics Peptidylprolyl Isomerase / metabolism RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Receptors, G-Protein-Coupled / genetics Receptors, G-Protein-Coupled / metabolism* Signal Transduction Spindle Apparatus Stem Cells / cytology* Stem Cells / metabolism |
| リソース情報 | |
| ショウジョウバエ | DGRC#119128 DGRC#119129 DGRC#119130 |