| RRC ID |
78619
|
| 著者 |
Alvarenga L, Saldanha JF, Stockler-Pinto MB, Fouque D, Soulage CO, Mafra D.
|
| タイトル |
Effects of resveratrol on inflammation and oxidative stress induced by the uremic toxin indoxyl sulfate in Murine macrophage-like RAW 264.7.
|
| ジャーナル |
Biochimie
|
| Abstract |
Indoxyl sulfate (IS) is a uremic toxin produced by the gut microbiota that commonly accumulates in patients with chronic kidney disease (CKD) and can be harmful. Resveratrol is a polyphenol with properties that attenuate oxidative stress and inflammation. This study aims to evaluate the effect of resveratrol against the damage caused by IS in RAW 264.7 murine macrophages. Cells were treated with 0, 250, 500 and 1000 μmol/L of IS, in the presence of 50 μmol/L of resveratrol. The mRNA and protein expressions of erythroid-related nuclear factor 2 (Nrf2) and nuclear factor kappa-B (NF-κB) were measured using rt-PCR and Western blot analysis, respectively. Malondialdehyde (MDA) and reactive oxygen species (ROS) levels were also analyzed. As a result, it was demonstrated that resveratrol induces the activation of the Nrf2 pathway that enhances cytoprotective response. IS upregulated the NF-κB expression and downregulated the Nrf2 expression. In contrast, resveratrol treatment significantly reduced the MDA and ROS production and inhibited the IS-induced expression of NF-κB in macrophage-like RAW 264.7. In conclusion, resveratrol can mitigate inflammation and oxidative stress caused by uremic toxins produced by the gut microbiota, such as IS.
|
| 巻・号 |
213
|
| ページ |
22-29
|
| 公開日 |
2023-10-1
|
| DOI |
10.1016/j.biochi.2023.05.001
|
| PII |
S0300-9084(23)00097-4
|
| PMID |
37142118
|
| MeSH |
Animals
Humans
Indican* / toxicity
Inflammation / drug therapy
Macrophages / metabolism
Mice
NF-E2-Related Factor 2 / metabolism
NF-kappa B* / metabolism
Oxidative Stress
Reactive Oxygen Species / metabolism
Resveratrol / pharmacology
Uremic Toxins
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
