| RRC ID |
78940
|
| 著者 |
Hanako Hagio, Wataru Koyama, Shiori Hosaka, Aysenur Deniz Song, Janchiv Narantsatsral, Koji Matsuda, Takashi Shimizu, Shoko Hososhima, Satoshi P Tsunoda, Hideki Kandori, Masahiko Hibi
|
| タイトル |
Optogenetic manipulation of neuronal and cardiomyocyte functions in zebrafish using microbial rhodopsins and adenylyl cyclases
|
| ジャーナル |
eLife
|
| Abstract |
Even though microbial photosensitive proteins have been used for optogenetics, their use should be optimized to precisely control cell and tissue functions in vivo. We exploited GtCCR4 and KnChR, cation channelrhodopsins from algae, BeGC1, a guanylyl cyclase rhodopsin from a fungus, and photoactivated adenylyl cyclases (PACs) from cyanobacteria (OaPAC) or bacteria (bPAC), to control cell functions in zebrafish. Optical activation of GtCCR4 and KnChR in the hindbrain reticulospinal V2a neurons, which are involved in locomotion, induced swimming behavior at relatively short latencies, whereas activation of BeGC1 or PACs achieved it at long latencies. Activation of GtCCR4 and KnChR in cardiomyocytes induced cardiac arrest, whereas activation of bPAC gradually induced bradycardia. KnChR activation led to an increase in intracellular Ca2+ in the heart, suggesting that depolarization caused cardiac arrest. These data suggest that these optogenetic tools can be used to reveal the function and regulation of zebrafish neurons and cardiomyocytes.
|
| 巻・号 |
12
|
| 公開日 |
2023-8-17
|
| DOI |
10.7554/elife.83975
|
| PMID |
37589546
|
| PMC |
PMC10435232
|
| MeSH |
Adenylyl Cyclases / genetics
Animals
Heart Arrest*
Myocytes, Cardiac*
Neurons
Optogenetics
Rhodopsins, Microbial
Zebrafish
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(chx10:Gal4) |
