Reference - Detail
| RRC ID | 78955 |
|---|---|
| Author | Kubo T, Yamagata Y, Matsusaka H, Toyoda A, Sato Y, Kumamaru T. |
| Title | MiRiQ Database: A Platform for In Silico Rice Mutant Screening. |
| Journal | Plant Cell Physiol |
| Abstract |
Genetic studies using mutant resources have significantly contributed to elucidating plant gene function. Massive mutant libraries sequenced by next-generation sequencing technology facilitate mutant identification and functional analysis of genes of interest. Here, we report the creation and release of an open-access database (https://miriq.agr.kyushu-u.ac.jp/index.php), called Mutation-induced Rice in Kyushu University (MiRiQ), designed for in silico mutant screening based on a whole-genome-sequenced mutant library. This database allows any user to easily find mutants of interest without laborious efforts such as large-scale screening by PCR. The initial version of the MiRiQ database (version 1.0) harbors a total of 1.6 million single-nucleotide variants (SNVs) and InDels of 721 M1 plants that were mutagenized by N-methyl-N-nitrosourea treatment of the rice cultivar Nipponbare (Oryza sativa ssp. japonica). The SNVs were distributed among 87% of all 35,630 annotated protein-coding genes of the Nipponbare genome and were predicted to induce missense and nonsense mutations. The MiRiQ database provides built-in tools, such as a search tool by keywords and JBrowse for mutation searches. Users can request mutant seeds in the M2 or M3 generations from a request form linked to this database. We believe that the availability of a wide range of gene mutations in this database will benefit the plant science community and breeders worldwide by accelerating functional genomic research and crop improvement. |
| Volume | 65(1) |
| Pages | 169-174 |
| Published | 2024-1-19 |
| DOI | 10.1093/pcp/pcad134 |
| PII | 7335587 |
| PMID | 37930817 |
| PMC | PMC10799713 |
| MeSH | Base Sequence Genes, Plant Genome, Plant / genetics Humans Mutation / genetics Oryza* / genetics |
| IF | 4.062 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Rice | Induced mutation lines |