RRC ID 79013
Author Steinmetz EL, Noh S, Klöppel C, Fuhr MF, Bach N, Raffael ME, Hildebrandt K, Wittling F, Jann D, Walldorf U.
Title Generation of Mutants from the 57B Region of Drosophila melanogaster.
Journal Genes (Basel)
Abstract The 57B region of Drosophila melanogaster includes a cluster of the three homeobox genes orthopedia (otp), Drosophila Retinal homeobox (DRx), and homeobrain (hbn). In an attempt to isolate mutants for these genes, we performed an EMS mutagenesis and isolated lethal mutants from the 57B region, among them mutants for otp, DRx, and hbn. With the help of two newly generated deletions from the 57B region, we mapped additional mutants to specific chromosomal intervals and identified several of these mutants from the 57B region molecularly. In addition, we generated mutants for CG15651 and RIC-3 by gene targeting and mutants for the genes CG9344, CG15649, CG15650, and ND-B14.7 using the CRISPR/Cas9 system. We determined the lethality period during development for most isolated mutants. In total, we analysed alleles from nine different genes from the 57B region of Drosophila, which could now be used to further explore the functions of the corresponding genes in the future.
Volume 14(11)
Published 2023-11-6
DOI 10.3390/genes14112047
PII genes14112047
PMID 38002990
PMC PMC10671637
MeSH Animals DNA-Binding Proteins / genetics Drosophila / genetics Drosophila Proteins* / genetics Drosophila melanogaster* / genetics Drosophila melanogaster* / metabolism Genes, Homeobox Homeodomain Proteins / genetics Homeodomain Proteins / metabolism ras Proteins / genetics
Resource
Drosophila DGRC#202812