| RRC ID |
79018
|
| 著者 |
González-Gutiérrez A, Gaete J, Esparza A, Toledo J, Köhler-Solis A, Sierralta J.
|
| タイトル |
Visualizing Monocarboxylates and Other Relevant Metabolites in the Ex Vivo Drosophila Larval Brain Using Genetically Encoded Sensors.
|
| ジャーナル |
J Vis Exp
|
| Abstract |
The high energy requirements of brains due to electrical activity are one of their most distinguishing features. These requirements are met by the production of ATP from glucose and its metabolites, such as the monocarboxylates lactate and pyruvate. It is still unclear how this process is regulated or who the key players are, particularly in Drosophila. Using genetically encoded Förster resonance energy transfer-based sensors, we present a simple method for measuring the transport of monocarboxylates and glucose in glial cells and neurons in an ex-vivo Drosophila larval brain preparation. The protocol describes how to dissect and adhere a larval brain expressing one of the sensors to a glass coverslip. We present the results of an entire experiment in which lactate transport was measured in larval brains by knocking down previously identified monocarboxylate transporters in glial cells. Furthermore, we demonstrate how to rapidly increase neuronal activity and track metabolite changes in the active brain. The described method, which provides all necessary information, can be used to analyze other Drosophila living tissues.
|
| 巻・号 |
(200)
|
| 公開日 |
2023-10-27
|
| DOI |
10.3791/65846
|
| PMID |
37955366
|
| MeSH |
Animals
Brain / metabolism
Drosophila* / metabolism
Glucose / metabolism
Lactic Acid* / metabolism
Pyruvic Acid / metabolism
|
| リソース情報 |
| ショウジョウバエ |
DGRC#117012 |
