RRC ID 79110
Author Krasovec G, Renaud C, Quéinnec É, Sasakura Y, Chambon JP.
Title Extrinsic apoptosis participates to tail regression during the metamorphosis of the chordate Ciona.
Journal Sci Rep
Abstract Apoptosis is a regulated cell death ubiquitous in animals defined by morphological features depending on caspases. Two regulation pathways are described, currently named the intrinsic and the extrinsic apoptosis. While intrinsic apoptosis is well studied and considered ancestral among metazoans, extrinsic apoptosis is poorly studied outside mammals. Here, we address extrinsic apoptosis in the urochordates Ciona, belonging to the sister group of vertebrates. During metamorphosis, Ciona larvae undergo a tail regression depending on tissue contraction, migration and apoptosis. Apoptosis begin at the tail tip and propagates towards the trunk as a polarized wave. We identified Ci-caspase 8/10 by phylogenetic analysis as homolog to vertebrate caspases 8 and 10 that are the specific initiator of extrinsic apoptosis. We detected Ci-caspase 8/10 expression in Ciona larvae, especially at the tail tip. We showed that chemical inhibition of Ci-caspase 8/10 leads to a delay of tail regression, and Ci-caspase 8/10 loss of function induced an incomplete tail regression. The specificity between apoptotic pathways and initiator caspase suggests that extrinsic apoptosis regulates cell death during the tail regression. Our study presents rare in vivo work on extrinsic apoptosis outside mammals, and contribute to the discussion on its evolutionary history in animals.
Volume 14(1)
Pages 5729
Published 2024-3-8
DOI 10.1038/s41598-023-48411-y
PII 10.1038/s41598-023-48411-y
PMID 38459045
PMC PMC10923776
MeSH Animals Apoptosis / genetics Caspase 8 / genetics Caspase 8 / metabolism Caspases / genetics Caspases / metabolism Ciona* Ciona intestinalis* / genetics Ciona intestinalis* / metabolism Mammals / metabolism Phylogeny
C.intestinalis / (O.japonicus) Wild C. int