RRC ID 79114
Author Takemoto K, Nishimura T, Kawasaki T, Imai Y, Levy K, Hart N, Olaya I, Burgess SM, Elkouby YM, Tanaka M, Sakai N.
Title In Vitro Storage of Functional Sperm at Room Temperature in Zebrafish and Medaka.
Journal Zebrafish
Abstract The longevity of sperm in teleost such as zebrafish and medaka is short when isolated even in saline-balanced solution at a physiological temperature. In contrast, some internal fertilizers exhibit the long-term storage of sperm, >10 months, in the female reproductive tract. This evidence implies that sperm in teleost possesses the ability to survive for a long time under suitable conditions; however, these conditions are not well understood. In this study, we show that the sperm of zebrafish can survive and maintain fertility in L-15-based storage medium supplemented with bovine serum albumin, fetal bovine serum, glucose, and lactic acid for 28 days at room temperature. The fertilized embryos developed to normal fertile adults. This storage medium was effective in medaka sperm stored for 7 days at room temperature. These results suggest that sperm from external fertilizer zebrafish and medaka has the ability to survive for at least 4 and 1 week, respectively, in the body fluid-like medium at a physiological temperature. This sperm storage method allows researchers to ship sperm by low-cost methods and to investigate key factors for motility and fertile ability in those sperm.
Volume 20(6)
Pages 229-235
Published 2023-12-1
DOI 10.1089/zeb.2023.0054
PMID 38010808
MeSH Animals Female Male Oryzias* / physiology Semen Semen Preservation* / methods Semen Preservation* / veterinary Sperm Motility / physiology Spermatozoa / physiology Temperature Zebrafish
IF 1.845
Resource
Medaka OK-Cab