論文 - 詳細
| RRC ID | 79158 |
|---|---|
| 著者 | Hagio H, Koyama W, Hosaka S, Song AD, Narantsatsral J, Matsuda K, Sugihara T, Shimizu T, Koyanagi M, Terakita A, Hibi M. |
| タイトル | Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells. |
| ジャーナル | Elife |
| Abstract |
G-protein-coupled receptors (GPCRs) transmit signals into cells depending on the G protein type. To analyze the functions of GPCR signaling, we assessed the effectiveness of animal G-protein-coupled bistable rhodopsins that can be controlled into active and inactive states by light application using zebrafish. We expressed Gq- and Gi/o-coupled bistable rhodopsins in hindbrain reticulospinal V2a neurons, which are involved in locomotion, or in cardiomyocytes. Light stimulation of the reticulospinal V2a neurons expressing Gq-coupled spider Rh1 resulted in an increase in the intracellular Ca2+ level and evoked swimming behavior. Light stimulation of cardiomyocytes expressing the Gi/o-coupled mosquito Opn3, pufferfish TMT opsin, or lamprey parapinopsin induced cardiac arrest, and the effect was suppressed by treatment with pertussis toxin or barium, suggesting that Gi/o-dependent regulation of inward-rectifier K+ channels controls cardiac function. These data indicate that these rhodopsins are useful for optogenetic control of GPCR-mediated signaling in zebrafish neurons and cardiomyocytes. |
| 巻・号 | 12 |
| 公開日 | 2023-8-17 |
| DOI | 10.7554/eLife.83974 |
| PII | 83974 |
| PMID | 37589544 |
| PMC | PMC10435233 |
| MeSH | Animals Myocytes, Cardiac* Neurons Optogenetics Potassium Channels, Inwardly Rectifying* Rhodopsin Zebrafish |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(5xUAS-hsp70l:Ace.UVOP-Flag-P2A-TagCFP, myl7:mCherry)nub41Tg Tg(5xUAS-hsp70l:Ace.BLOP-Flag-P2A-TagCFP, myl7:mCherry)nub42Tg Tg(5xUAS-hsp70l:Pxu.Rh3-Flag-P2A-TagCFP; myl7:mCherry)nub43Tg Tg(5xUAS-hsp70l:Ast.Opn3-Flag-P2A-TagCFP, myl7:mCherry)nub44Tg Tg(5xUAS-hsp70l:Tru.TMT-Flag-P2A-TagCFP, myl7:mCherry)nub45Tg Tg(5xUAS-hsp70l:Lca.PP-Flag-P2A-TagCFP, myl7:mCherry)nub46Tg Tg(5xUAS-hsp70l:parapinopsina-Flag-P2A-TagCFP, myl7:mCherry)nub47Tg Tg(5xUAS-hsp70l:parapinopsinb-Flag-P2A-TagCFP, myl7:mCherry)nub48Tg Tg(5xUAS-hsp70l:GtACR1-EYFP, myl7:mCherry)nub53Tg Tg(5xUAS-hsp70l:ChrimsonR-tdTomato)nub119Tg TgBAC(vsx2:GAL4FF) nns18Tg Tg(UAS:ChRWR-EGFP)js3Tg Tg(5xUAS-hsp70l:GCaMP6s) nkUAShspzGCaMP6s13aTg Tg(myl7:GAL4FF)nub38Tg Tg(5xUAS-hsp70l:Had.Rh1-Flag-P2A-TagCFP, myl7:mCherry)nub39Tg Tg(5xUAS-hsp70l:Had.Rh1[S186F]-Flag-P2A-TagCFP, myl7:mCherry)nub40Tg |