| RRC ID |
79158
|
| 著者 |
Hagio H, Koyama W, Hosaka S, Song AD, Narantsatsral J, Matsuda K, Sugihara T, Shimizu T, Koyanagi M, Terakita A, Hibi M.
|
| タイトル |
Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells.
|
| ジャーナル |
Elife
|
| Abstract |
G-protein-coupled receptors (GPCRs) transmit signals into cells depending on the G protein type. To analyze the functions of GPCR signaling, we assessed the effectiveness of animal G-protein-coupled bistable rhodopsins that can be controlled into active and inactive states by light application using zebrafish. We expressed Gq- and Gi/o-coupled bistable rhodopsins in hindbrain reticulospinal V2a neurons, which are involved in locomotion, or in cardiomyocytes. Light stimulation of the reticulospinal V2a neurons expressing Gq-coupled spider Rh1 resulted in an increase in the intracellular Ca2+ level and evoked swimming behavior. Light stimulation of cardiomyocytes expressing the Gi/o-coupled mosquito Opn3, pufferfish TMT opsin, or lamprey parapinopsin induced cardiac arrest, and the effect was suppressed by treatment with pertussis toxin or barium, suggesting that Gi/o-dependent regulation of inward-rectifier K+ channels controls cardiac function. These data indicate that these rhodopsins are useful for optogenetic control of GPCR-mediated signaling in zebrafish neurons and cardiomyocytes.
|
| 巻・号 |
12
|
| 公開日 |
2023-8-17
|
| DOI |
10.7554/eLife.83974
|
| PII |
83974
|
| PMID |
37589544
|
| PMC |
PMC10435233
|
| MeSH |
Animals
Myocytes, Cardiac*
Neurons
Optogenetics
Potassium Channels, Inwardly Rectifying*
Rhodopsin
Zebrafish
|
| リソース情報 |
| ゼブラフィッシュ |
TgBAC(vsx2:GAL4FF) nns18Tg
Tg(UAS:ChRWR-EGFP)js3Tg
Tg(5xUAS-hsp70l:GCaMP6s) nkUAShspzGCaMP6s13aTg
Tg(myl7:GAL4FF)nub38Tg
Tg(5xUAS-hsp70l:Had.Rh1-Flag-P2A-TagCFP, myl7:mCherry)nub39Tg
Tg(5xUAS-hsp70l:Had.Rh1[S186F]-Flag-P2A-TagCFP, myl7:mCherry)nub40Tg
Tg(5xUAS-hsp70l:Ace.UVOP-Flag-P2A-TagCFP, myl7:mCherry)nub41Tg
Tg(5xUAS-hsp70l:Ace.BLOP-Flag-P2A-TagCFP, myl7:mCherry)nub42Tg
Tg(5xUAS-hsp70l:Pxu.Rh3-Flag-P2A-TagCFP; myl7:mCherry)nub43Tg
Tg(5xUAS-hsp70l:Ast.Opn3-Flag-P2A-TagCFP, myl7:mCherry)nub44Tg
Tg(5xUAS-hsp70l:Tru.TMT-Flag-P2A-TagCFP, myl7:mCherry)nub45Tg
Tg(5xUAS-hsp70l:Lca.PP-Flag-P2A-TagCFP, myl7:mCherry)nub46Tg
Tg(5xUAS-hsp70l:parapinopsina-Flag-P2A-TagCFP, myl7:mCherry)nub47Tg
Tg(5xUAS-hsp70l:parapinopsinb-Flag-P2A-TagCFP, myl7:mCherry)nub48Tg
Tg(5xUAS-hsp70l:GtACR1-EYFP, myl7:mCherry)nub53Tg
Tg(5xUAS-hsp70l:ChrimsonR-tdTomato)nub119Tg |
