RRC ID 79176
Author Furuno K, Suzuki K, Sakai S.
Title Transduction and Genome Editing of the Heart with Adeno-Associated Viral Vectors Loaded onto Electrospun Polydioxanone Nonwoven Fabrics.
Journal Biomolecules
Abstract In this study, we introduce electrospun polydioxanone (PDO) nonwoven fabrics as a platform for the delivery of adeno-associated virus (AAV) vectors for transduction and genome editing by adhering them to organ surfaces, including the heart. AAV vectors were loaded onto the PDO fabrics by soaking the fabrics in a solution containing AAV vectors. In vitro, the amount of AAV vectors loaded onto the fabrics could be adjusted by changing their concentration in the solution, and the number of cells expressing the green fluorescent protein (GFP) encoded by the AAV vectors increased in correlation with the increasing amount of loaded AAV vectors. In vivo, both transduction and genome editing resulted in the observation of GFP expression around AAV vector-loaded PDO fabrics attached to the surfaces of mouse hearts, indicating effective transduction and expression at the target site. These results demonstrate the great potential of electrospun PDO nonwoven fabrics carrying therapeutic AAV vectors for gene therapy.
Volume 14(4)
Published 2024-4-22
DOI 10.3390/biom14040506
PII biom14040506
PMID 38672522
PMC PMC11047894
MeSH Animals Dependovirus* / genetics Gene Editing* / methods Genetic Therapy / methods Genetic Vectors* / genetics Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HEK293 Cells Humans Mice Myocardium / metabolism Polydioxanone* / chemistry Transduction, Genetic / methods
Human and Animal Cells Hep G2(RCB1886)