論文 - 詳細
| RRC ID | 79205 |
|---|---|
| 著者 | Zou J, Anai S, Ota S, Ishitani S, Oginuma M, Ishitani T. |
| タイトル | Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB. |
| ジャーナル | Nat Commun |
| Abstract |
In vertebrate embryos, the canonical Wnt ligand primes the formation of dorsal organizers that govern dorsal-ventral patterns by secreting BMP antagonists. In contrast, in Drosophila embryos, Toll-like receptor (Tlr)-mediated NFκB activation initiates dorsal-ventral patterning, wherein Wnt-mediated negative feedback regulation of Tlr/NFκB generates a BMP antagonist-secreting signalling centre to control the dorsal-ventral pattern. Although both Wnt and BMP antagonist are conserved among species, the involvement of Tlr/NFκB and feedback regulation in vertebrate organizer formation remains unclear. By imaging and genetic modification, we reveal that a negative feedback loop between canonical and non-canonical Wnts and Tlr4/NFκB determines the size of zebrafish organizer, and that Tlr/NFκB and Wnts switch initial cue and feedback mediator roles between Drosophila and zebrafish. Here, we show that canonical Wnt signalling stimulates the expression of the non-canonical Wnt5b ligand, activating the Tlr4 receptor to stimulate NFκB-mediated transcription of the Wnt antagonist frzb, restricting Wnt-dependent dorsal organizer formation. |
| 巻・号 | 14(1) |
| ページ | 7194 |
| 公開日 | 2023-11-8 |
| DOI | 10.1038/s41467-023-42963-3 |
| PII | 10.1038/s41467-023-42963-3 |
| PMID | 37938219 |
| PMC | PMC10632484 |
| MeSH | Animals Drosophila Feedback Ligands NF-kappa B* Wnt Signaling Pathway Zebrafish* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(6xTcf/LefBS-miniP:d2EGFP)(Tg(OTM:d2EGFP)) Tg(6xNFκB BS-tkP:D2EGFP) rela-A |