論文 - 詳細
| RRC ID | 79206 |
|---|---|
| 著者 | Sone R, Fujimaki S, Kawahara A. |
| タイトル | Efficient detection of single nucleotide variants in targeted genomic loci. |
| ジャーナル | Dev Growth Differ |
| Abstract |
Single nucleotide variants (SNVs), including single nucleotide polymorphisms, are often associated with morphological and/or physiological abnormalities in various organisms. Targeted genomic DNA can be amplified and directly sequenced to detect these mutations, but this method is relatively time consuming and expensive. We recently established the heteroduplex mobility assay to detect genetic mutations as an easy, low-cost method in genome editing, but detecting such small genetic differences remains difficult. Here, we developed a new, simple method to detect single nucleotide changes in the zebrafish genome by polymerase chain reaction (PCR) and electrophoresis. We first designed a specific single stranded DNA with four tandem guanine nucleotides inserted beside the mutation site, called guanine-inserted primer (GIP). When reannealing, hybridized complexes of GIP and PCR amplicons with or without 1-bp-mutated alleles form different bulge structures, presumably leading to different mobilities on a polyacrylamide gel. This GIP-interacting mobility assay is easy to use; therefore, it could contribute to the detection of SNVs in any organism. |
| 巻・号 | 66(2) |
| ページ | 172-177 |
| 公開日 | 2024-2-1 |
| DOI | 10.1111/dgd.12910 |
| PMID | 38243758 |
| MeSH | Animals DNA* / genetics Genomics Mutation Nucleotides Zebrafish* / genetics |
| IF | 1.723 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | spns2 |