| RRC ID |
79260
|
| Author |
Maekawa Y, Matsui K, Okamoto K, Shimasaki T, Ohtsuka H, Tani M, Ihara K, Aiba H.
|
| Title |
Identification of plb1 mutation that extends longevity via activating Sty1 MAPK in Schizosaccharomyces pombe.
|
| Journal |
Mol Genet Genomics
|
| Abstract |
To understand the lifespan of higher organisms, including humans, it is important to understand lifespan at the cellular level as a prerequisite. So, fission yeast is a good model organism for the study of lifespan. To identify the novel factors involved in longevity, we are conducting a large-scale screening of long-lived mutant strains that extend chronological lifespan (cell survival in the stationary phase) using fission yeast. One of the newly acquired long-lived mutant strains (No.98 mutant) was selected for analysis and found that the long-lived phenotype was due to a missense mutation (92Phe → Ile) in the plb1+ gene. plb1+ gene in fission yeast is a nonessential gene encoding a homolog of phospholipase B, but its functions under normal growth conditions, as well as phospholipase B activity, remain unresolved. Our analysis of the No.98 mutant revealed that the plb1 mutation reduces the integrity of the cellular membrane and cell wall and activates Sty1 via phosphorylation.
|
| Volume |
299(1)
|
| Pages |
20
|
| Published |
2024-2-29
|
| DOI |
10.1007/s00438-024-02107-8
|
| PII |
10.1007/s00438-024-02107-8
|
| PMID |
38424265
|
| MeSH |
Gene Expression Regulation, Fungal
Humans
Longevity / genetics
Lysophospholipase / genetics
Lysophospholipase / metabolism
Mutation
Schizosaccharomyces* / metabolism
Schizosaccharomyces pombe Proteins* / genetics
Schizosaccharomyces pombe Proteins* / metabolism
|
| IF |
2.797
|
| Resource |
| Yeast |
|
