論文 - 詳細
| RRC ID | 79365 |
|---|---|
| 著者 | Xue XF, Zhnag MM, Sun JF, Li X, Wu QM, Yin Z, Yang WH, Ni B, Hu LF, Zhou DS, Lu RF, Zhang YQ. |
| タイトル | H-NS Represses Biofilm Formation and c-di-GMP Synthesis in Vibrio parahaemolyticus. |
| ジャーナル | Biomed Environ Sci |
| Abstract |
OBJECTIVE:This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in Vibrio parahaemolyticus RIMD2210633. METHODS:Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay. RESULTS:The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in V. parahaemolyticus RIMD2210633. H-NS can bind the upstream promoter-proximal DNA regions of scrA, scrG, VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism. CONCLUSION:One of the mechanisms by which H-NS represses the biofilm formation by V. parahaemolyticus RIMD2210633 may be via repression of the production of intracellular c-di-GMP. |
| 巻・号 | 35(9) |
| ページ | 821-829 |
| 公開日 | 2022-9-20 |
| DOI | 10.3967/bes2022.106 |
| PMID | 36189997 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism Biofilms Cyclic GMP / analogs & derivatives Gene Expression Regulation, Bacterial Gentian Violet Histones / genetics Histones / metabolism Vibrio parahaemolyticus* / genetics |
| リソース情報 | |
| 病原細菌 | V. parahaemolyticus |