論文 - 詳細
| RRC ID | 79399 |
|---|---|
| 著者 | Zhang Y, Zhang T, Qiu Y, Zhang M, Lu X, Yang W, Hu L, Zhou D, Gao B, Lu R. |
| タイトル | Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation. |
| ジャーナル | Curr Microbiol |
| Abstract |
Vibrio parahaemolyticus, the leading cause of bacterial seafood-associated gastroenteritis, can form biofilms. In this work, the gene expression profiles of V. parahaemolyticus during biofilm formation were investigated by transcriptome sequencing. A total of 183, 503, and 729 genes were significantly differentially expressed in the bacterial cells at 12, 24 and 48 h, respectively, compared with that at 6 h. Of these, 92 genes were consistently activated or repressed from 6 to 48 h. The genes involved in polar flagellum, chemotaxis, mannose-sensitive haemagglutinin type IV pili, capsular polysaccharide, type III secretion system 1 (T3SS1), T3SS2, thermostable direct hemolysin (TDH), type VI secretion system 1 (T6SS1) and T6SS2 were downregulated, whereas those involved in V. parahaemolyticus pathogenicity island (Vp-PAI) (except for T3SS2 and TDH) and membrane fusion proteins were upregulated. Three extracellular protease genes (vppC, prtA and VPA1071) and a dozen of outer membrane protein encoding genes were also significantly differentially expressed during biofilm formation. In addition, five putative c-di-GMP metabolism-associated genes were significantly differentially expressed, which may account for the drop in c-di-GMP levels after the beginning of biofilm formation. Moreover, many putative regulatory genes were significantly differentially expressed, and more than 1000 putative small non-coding RNAs were detected, suggesting that biofilm formation was tightly regulated by complex regulatory networks. The data provided a global view of gene expression profiles during biofilm formation, showing that the significantly differentially expressed genes were involved in multiple cellular pathways, including virulence, biofilm formation, metabolism, and regulation. |
| 巻・号 | 80(12) |
| ページ | 371 |
| 公開日 | 2023-10-14 |
| DOI | 10.1007/s00284-023-03425-7 |
| PII | 10.1007/s00284-023-03425-7 |
| PMID | 37838636 |
| MeSH | Bacterial Proteins / genetics Bacterial Proteins / metabolism Biofilms Gene Expression Regulation, Bacterial Humans Transcriptome Vibrio Infections* / microbiology Vibrio parahaemolyticus* / genetics Virulence / genetics Virulence Factors / genetics |
| IF | 1.746 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原細菌 | |