| RRC ID |
79448
|
| 著者 |
Minamitani T, Ariga H, Matsumoto K.
|
| タイトル |
Adhesive defect in extracellular matrix tenascin-X-null fibroblasts: a possible mechanism of tumor invasion.
|
| ジャーナル |
Biol Pharm Bull
|
| Abstract |
Extracellular matrix tenascin-X (TNX)-null mice, generated by disruption of the Tnx gene, display augmented invasion and metastasis of B16-BL6 melanoma tumor cells due to increased activities of matrix metalloproteinase (MMP)-2 and MMP-9. In this study, we investigated cell-matrix and cell-cell adhesions using TNX-null fibroblasts and wild-type fibroblasts. TNX-null fibroblasts exhibited a decreased attachment to fibronectin compared with that of wild-type fibroblasts. B16 melanoma cells were cocultured with wild-type or TNX-null fibroblasts, and the adhesion of B16 melanoma to the fibroblasts was assessed. B16 melanoma cells on wild-type fibroblasts proliferated and spread out in a horizontal direction, whereas those on TNX-null fibroblasts overlapped each other rather than migrating horizontally. These overlapping B16 melanoma cells on TNX-null fibroblasts peeled off faster than those on wild-type fibroblasts. To determine whether the decreased cell-matrix and cell-cell adhesions on TNX-null fibroblasts were due to increased MMP activity, the activities of MMPs in wild-type and TNX-null fibroblasts were compared by gelatinolytic assays. The analysis of MMPs from conditioned media demonstrated that almost the same levels of MMP activities were detected between wild-type and TNX-null fibroblasts. However, contrary to our expectations the activities of MMPs from conditioned media of B16 melanoma cells cocultured on TNX-null fibroblasts were rather reduced than those of B16 melanoma cells cocultured on wild-type. We concluded that the absence of TNX in the extracellular environment might play an important role in enhancement of the detachment of B16 melanoma cells.
|
| 巻・号 |
25(11)
|
| ページ |
1472-5
|
| 公開日 |
2002-11-1
|
| DOI |
10.1248/bpb.25.1472
|
| PMID |
12419962
|
| MeSH |
Animals
Cell Adhesion / genetics
Coculture Techniques / methods
Extracellular Matrix / genetics
Extracellular Matrix / metabolism*
Fibroblasts / metabolism*
Melanoma, Experimental / genetics
Melanoma, Experimental / metabolism*
Melanoma, Experimental / pathology*
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Inbred ICR
Mice, Knockout
Neoplasm Invasiveness / genetics
Neoplasm Invasiveness / pathology
Tenascin / deficiency*
Tenascin / genetics*
Tumor Cells, Cultured
|
| IF |
1.863
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma/lacZ(RCB1284) |
