RRC ID 80036
Author Ninomiya K, Kaneda K, Kawashima S, Miyachi Y, Ogino C, Shimizu N.
Title Cell-SELEX based selection and characterization of DNA aptamer recognizing human hepatocarcinoma.
Journal Bioorg Med Chem Lett
Abstract Single-stranded DNA aptamers recognizing human hepatocarcinoma were isolated by means of a systematic evolution of ligands by exponential enrichment using whole cells as targets (cell-SELEX). After 11 rounds of cell-SELEX procedure using human hepatoma HepG2 cells as targets and human normal hepatocyte cells as counterparts, 12 independent DNA aptamer candidate sequences were obtained. The specific interaction between selected DNA aptamers and targeted cell was confirmed. Dissociation constants of the 12 sequences obtained were also estimated in the range of 19-450nM. Moreover, the consensus secondary structure was found in the isolated aptamers, which was responsible to the recognition of HepG2 cells.
Volume 23(6)
Pages 1797-802
Published 2013-3-15
DOI 10.1016/j.bmcl.2013.01.040
PII S0960-894X(13)00073-5
PMID 23403083
MeSH Aptamers, Nucleotide / chemistry* Aptamers, Nucleotide / metabolism Base Sequence Carcinoma, Hepatocellular Cell Separation* DNA, Single-Stranded / chemistry DNA, Single-Stranded / metabolism* Flow Cytometry Hep G2 Cells Humans Liver Neoplasms Microscopy, Fluorescence Nucleic Acid Conformation SELEX Aptamer Technique
IF 2.572
Resource
Human and Animal Cells Hep G2(RCB1648)