| RRC ID |
80183
|
| Author |
Yang M, Zhang X, Ma S, Zhang Q, Peng C, Fan H, Dai L, Li J, Cheng L.
|
| Title |
Shumkonia mesophila gen. nov., sp. nov., a novel representative of Shumkoniaceae fam. nov. and its potentials for extracellular polymeric substances formation and sulfur metabolism revealed by genomic analysis.
|
| Journal |
Antonie Van Leeuwenhoek
|
| Abstract |
A microaerophilic, mesophilic, chemoorganoheterotrophic bacterium, designated Y-P2T, was isolated from oil sludge enrichment in China. Cells of the strain were Gram-stain-negative, non-motile, non-spore-forming, rod-shaped or slightly curved with 0.8-3.0 µm in length and 0.4-0.6 µm in diameter. The strain Y-P2T grew optimally at 25 °C (range from 15 to 30 °C) and pH 7.0 (range from pH 6.0 to 7.5) without NaCl. The major cellular fatty acids were C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The main polar liquids of strain Y-P2T comprised phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The respiratory quinone was Q-10. Acetate and H2 were the fermentation products of glucose. The DNA G + C content was 66.0%. Strain Y-P2T shared the highest 16S rRNA gene sequence similarity (90.3-90.6%) with species within Oceanibaculum of family Thalassobaculaceae in Rhodospirillales. Phylogenetic analyses based on 16S rRNA gene sequences and genomes showed that strain Y-P2T formed a distinct evolutionary lineage within the order Rhodospirillales. On the basis of phenotypic, phylogenetic and phylogenomic data, we propose that strain Y-P2T represents a novel species in a novel genus, for which Shumkonia mesophila gen. nov., sp. nov., within a new family Shumkoniaceae fam. nov. The type strain is Y-P2T (= CCAM 826 T = JCM 34766 T).
|
| Volume |
116(12)
|
| Pages |
1359-1374
|
| Published |
2023-12-1
|
| DOI |
10.1007/s10482-023-01878-1
|
| PII |
10.1007/s10482-023-01878-1
|
| PMID |
37843737
|
| MeSH |
Bacterial Typing Techniques
DNA, Bacterial / genetics
Extracellular Polymeric Substance Matrix*
Fatty Acids / chemistry
Genomics
Phospholipids* / chemistry
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sulfur
Ubiquinone / chemistry
|
| Resource |
| General Microbes |
JCM 34766 |
