| RRC ID |
80189
|
| 著者 |
Sun P, Li X, Shi W, Zhang L, Li M, Su L, Qin C.
|
| タイトル |
Lactococcus intestinalis sp. nov., a new lactic acid bacterium isolated from intestinal contents in Alzheimer's disease mice.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
A gram-positive, facultatively anaerobic, and coccoid or ovoid-shaped bacterium designated M2458T was isolated from the intestinal contents of APPswe/PSΔE9 mouse model of Alzheimer's disease. With the polyphasic approach, the taxonomic position of the novel isolate was confirmed. Strain M2458T grew well at 37 °C on YCFA agar. Strain M2458T belongs to the family Streptococcaceae and class Bacilli, and it is closed to Lactococcus formosensis NBRC 109475T (97.59% sequence similarity) according to its 16S rRNA gene sequence. In a comparison of two housekeeping genes, rpoA and rpoB, strain M2458T was found to be well separated from Lactococcus formosensis NBRC 109475T. On the basis of whole genome sequences, the DNA G+C content was 38.29 mol%. The phylogentic analysis of the whole genome showed that a different branch was clearly formed in the phylogenetic tree of strain M2458T compared to other strains in the genus Lactococcus. A total of eight genes in strain M2458T are involved in the 'neurodegenerative disease' pathway, which involves an annotated protein (glyceraldehyde 3-phosphate dehydrogenase) involved in Alzheimer's disease. In terms of average nucleotide identity and digital DNA-DNA hybridization, strain M2458T was identified as a novel species of the genus Lacococcus. The major fatty acids (> 10% of the total fatty acids) were C18:1ω9c (39.68%), C16:0 (13.26%) and C18:1ω7c (11.52%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid. As a result of its genotypic and phenotypic characteristics, strain M2458T was considered to be a new species within the genus Lactococcus; the name Lactococcus intestinalis sp. nov. has been proposed, with type strain M2458T (=JCM 35706 = CGMCC 1.60066).
|
| 巻・号 |
116(5)
|
| ページ |
425-433
|
| 公開日 |
2023-5-1
|
| DOI |
10.1007/s10482-023-01814-3
|
| PII |
10.1007/s10482-023-01814-3
|
| PMID |
36786966
|
| MeSH |
Alzheimer Disease*
Animals
Bacterial Typing Techniques
DNA, Bacterial / genetics
Fatty Acids
Gastrointestinal Contents
Lactic Acid
Lactococcus
Mice
Nucleic Acid Hybridization
Phospholipids
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| リソース情報 |
| 一般微生物 |
JCM 35706 |
