論文 - 詳細
| RRC ID | 80191 |
|---|---|
| 著者 | Han S, Tang R, Yang S, Xie CJ, Narsing Rao MP, Rensing C, Liu GH, Zhou SG. |
| タイトル | Geothrix oryzisoli sp. nov., a ferric iron-reducing bacterium isolated from paddy soil. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
An anaerobic, Gram-staining-negative, rod-shaped, Fe(III)-reducing strain, designated SG189T, was isolated from paddy soil in Fujian Province, China. Growth occurred at 20-35 ℃ (optimum 30 ℃), pH 6.5-8.0 (optimum 7.0) and 0-0.2% (w/v) NaCl (optimum 0%). The strain SG189T showed the highest 16S rRNA sequences similarities to the type strains of Geothrix fermentans DSM 14018T (98.9%), "Geothrix terrae" SG184T (99.0%) and "Geothrix alkalitolerans" SG263T (99.3%). ANI and dDDH values between strain SG189T and the most closely related Geothrix species were 86.5-87.1% and 31.5-32.9%, which lower than the cut-off values (ANI 95-96% and dDDH 70%) for prokaryotic species delineation. Further, genome-based phylogenomic trees constructed using 81 core genes (UBCG2) and 120 conserved genes (GTDB) showed that strain SG189T formed a clade with members of the genus Geothrix. The menaquinone was shown to be MK-8, and the major fatty acids were iso-C15:0 and iso-C13:0 3OH. The genomic DNA G + C content was 68.2%. Additionally, we found that strain SG189T possessed ability to reduce ferric iron, and strain SG189T could reduce 10 mM of ferric citrate in 10 days with lactate as the sole electron donor. Based on the observed physiological and biochemical properties, chemotaxonomic characteristics, ANI and dDDH values, SG189T represents a novel species of the genus Geothrix, for which the name Geothrix oryzisoli sp. nov. is proposed. The type strain is SG189T (= GDMCC 1.3408T = JCM 39324T). |
| 巻・号 | 116(5) |
| ページ | 477-486 |
| 公開日 | 2023-5-1 |
| DOI | 10.1007/s10482-023-01817-0 |
| PII | 10.1007/s10482-023-01817-0 |
| PMID | 36897496 |
| MeSH | Bacteria / genetics Bacterial Typing Techniques DNA, Bacterial / genetics Fatty Acids / chemistry Ferric Compounds* Iron Phospholipids* / chemistry Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 39324 |