論文 - 詳細
| RRC ID | 80252 |
|---|---|
| 著者 | Han DM, Baek JH, Choi DG, Jin MS, Jeon CO. |
| タイトル | Chitinophaga horti sp. nov., Isolated from Garden Soil. |
| ジャーナル | Curr Microbiol |
| Abstract |
A strictly aerobic Gram-negative bacterium, designated R8T, isolated from garden soil in South Korea was subjected to a taxonomic study. The cells were non-spore-forming, oxidase-positive and catalase-negative, and non-motile rods (without flagella). Growth was observed between 10 °C and 40 °C (optimum, 30 °C) and between pH 6.0 and 9.0 (optimum, pH 7.0) and in the presence of 0%-1.5% (w/v) NaCl (optimum, 0%). The G + C content of the genomic DNA was 49.9% and the major isoprenoid quinone was found to be menaquinone-7. The major fatty acids of strain R8T were iso-C15:0, C16:1 ω5c, and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1 ω7c/ω6c). Phosphatidylethanolamine was identified as a major polar lipid. Comparative 16S rRNA gene sequence analysis showed that strain R8T had the highest 16S rRNA gene sequence similarity of 98.3% with Chitinophaga sedimenti TFL-3 T. Phylogenetic analyses using 16S rRNA gene sequences and concatenated 92 marker protein sequences revealed that strain R8T formed a robust phylogenetic lineage with C. sedimenti within the genus Chitinophaga. Average nucleotide identity and digital DNA-DNA hybridization values of strain R8T to Chitinophaga species were less than 77.9% and 21.1%, respectively. The phenotypic, phylogenetic, and chemotaxonomic properties support that strain R8T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga horti sp. nov. is proposed. The type strain is R8T (= KACC 19895 T = JCM 33215 T). |
| 巻・号 | 80(4) |
| ページ | 116 |
| 公開日 | 2023-2-25 |
| DOI | 10.1007/s00284-023-03222-2 |
| PII | 10.1007/s00284-023-03222-2 |
| PMID | 36841905 |
| MeSH | Bacterial Typing Techniques DNA, Bacterial / chemistry DNA, Bacterial / genetics Fatty Acids / chemistry Gammaproteobacteria* / genetics Gardens* Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Soil Microbiology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 33215 |