RRC ID 80301
Author Sekiguchi Y, Teramoto K, Tourlousse DM, Ohashi A, Hamajima M, Miura D, Yamada Y, Iwamoto S, Tanaka K.
Title A large-scale genomically predicted protein mass database enables rapid and broad-spectrum identification of bacterial and archaeal isolates by mass spectrometry.
Journal Genome Biol
Abstract MALDI-TOF MS-based microbial identification relies on reference spectral libraries, which limits the screening of diverse isolates, including uncultured lineages. We present a new strategy for broad-spectrum identification of bacterial and archaeal isolates by MALDI-TOF MS using a large-scale database of protein masses predicted from nearly 200,000 publicly available genomes. We verify the ability of the database to identify microorganisms at the species level and below, achieving correct identification for > 90% of measured spectra. We further demonstrate its utility by identifying uncultured strains from mouse feces with metagenomics, allowing the identification of new strains by customizing the database with metagenome-assembled genomes.
Volume 24(1)
Pages 257
Published 2023-12-5
DOI 10.1186/s13059-023-03096-4
PII 10.1186/s13059-023-03096-4
PMID 38049850
PMC PMC10696839
MeSH Animals Archaea* / genetics Bacteria* / genetics Databases, Factual Mice Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Resource
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