Reference - Detail
| RRC ID | 80301 |
|---|---|
| Author | Sekiguchi Y, Teramoto K, Tourlousse DM, Ohashi A, Hamajima M, Miura D, Yamada Y, Iwamoto S, Tanaka K. |
| Title | A large-scale genomically predicted protein mass database enables rapid and broad-spectrum identification of bacterial and archaeal isolates by mass spectrometry. |
| Journal | Genome Biol |
| Abstract |
MALDI-TOF MS-based microbial identification relies on reference spectral libraries, which limits the screening of diverse isolates, including uncultured lineages. We present a new strategy for broad-spectrum identification of bacterial and archaeal isolates by MALDI-TOF MS using a large-scale database of protein masses predicted from nearly 200,000 publicly available genomes. We verify the ability of the database to identify microorganisms at the species level and below, achieving correct identification for > 90% of measured spectra. We further demonstrate its utility by identifying uncultured strains from mouse feces with metagenomics, allowing the identification of new strains by customizing the database with metagenome-assembled genomes. |
| Volume | 24(1) |
| Pages | 257 |
| Published | 2023-12-5 |
| DOI | 10.1186/s13059-023-03096-4 |
| PII | 10.1186/s13059-023-03096-4 |
| PMID | 38049850 |
| PMC | PMC10696839 |
| MeSH | Animals Archaea* / genetics Bacteria* / genetics Databases, Factual Mice Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 17 |
| Altmetric score changes over past 6months | 0.0 |
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| General Microbes | JCM 10133 JCM 14653 JCM 10043 JCM 9316 JCM 10044 JCM 13029 JCM 1217 JCM 11019 JCM 30898 JCM12577 JCM 13669 JCM 11387 JCM 12579 JCM12578 JCM 13670 JCM 12099 JCM 12098 JCM 12100 JCM 13213 JCM 13215 JCM 13216 |