| RRC ID |
80301
|
| 著者 |
Sekiguchi Y, Teramoto K, Tourlousse DM, Ohashi A, Hamajima M, Miura D, Yamada Y, Iwamoto S, Tanaka K.
|
| タイトル |
A large-scale genomically predicted protein mass database enables rapid and broad-spectrum identification of bacterial and archaeal isolates by mass spectrometry.
|
| ジャーナル |
Genome Biol
|
| Abstract |
MALDI-TOF MS-based microbial identification relies on reference spectral libraries, which limits the screening of diverse isolates, including uncultured lineages. We present a new strategy for broad-spectrum identification of bacterial and archaeal isolates by MALDI-TOF MS using a large-scale database of protein masses predicted from nearly 200,000 publicly available genomes. We verify the ability of the database to identify microorganisms at the species level and below, achieving correct identification for > 90% of measured spectra. We further demonstrate its utility by identifying uncultured strains from mouse feces with metagenomics, allowing the identification of new strains by customizing the database with metagenome-assembled genomes.
|
| 巻・号 |
24(1)
|
| ページ |
257
|
| 公開日 |
2023-12-5
|
| DOI |
10.1186/s13059-023-03096-4
|
| PII |
10.1186/s13059-023-03096-4
|
| PMID |
38049850
|
| PMC |
PMC10696839
|
| MeSH |
Animals
Archaea* / genetics
Bacteria* / genetics
Databases, Factual
Mice
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
|
| リソース情報 |
| 一般微生物 |
JCM 10133
JCM 14653
JCM 10043
JCM 9316
JCM 10044
JCM 13029
JCM 1217
JCM 11019
JCM 30898
JCM12577
JCM 13669
JCM 11387
JCM 12579
JCM12578
JCM 13670
JCM 12099
JCM 12098
JCM 12100
JCM 13213
JCM 13215
JCM 13216 |
