Reference - Detail
| RRC ID | 80416 |
|---|---|
| Author | Allen-Vercoe E, Daigneault MC, Vancuren SJ, Cochrane K, O'Neal LL, Sankaranarayanan K, Lawson PA. |
| Title | Holtiella tumoricola gen. nov. sp. nov., isolated from a human clinical sample. |
| Journal | Int J Syst Evol Microbiol |
| Abstract |
The diversity of bacteria associated with biopsy material obtained from patients with colorectal cancer was investigated using culture techniques. A novel bacterium, strain CC70AT, was isolated by diluting a sample of homogenized tissue in anaerobic medium, and then plating to yield a pure culture. Strain CC70AT was a Gram-positive, strictly anaerobic, motile, rod-shaped bacterium. Formate, but not acetate, was a fermentative end-product from growth in peptone-yeast extract and peptone-yeast-glucose broth. The G+C content of DNA from strain CC70AT was 34.9 mol%. 16S rRNA gene sequence analysis revealed that the isolate was part of the phylum Bacillota. The closest described relatives of strain CC70AT were Cellulosilyticum lentocellum (93.3 %) and Cellulosilyticum ruminicola (93.3 and 91.9% sequence similarity across 16S rRNA gene, respectively). According to the data obtained in this work, strain CC70AT represents a novel bacterium belonging to a new genus for which the name Holtiella tumoricola gen. nov., sp. nov. is proposed. The type strain for our described novel species is CC70AT (=DSM 27931T= JCM 30568T). |
| Volume | 73(7) |
| Published | 2023-7-1 |
| DOI | 10.1099/ijsem.0.005958 |
| PMID | 37436807 |
| MeSH | Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids* / chemistry Gram-Positive Bacteria Humans Peptones* Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
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| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 30568 |