| RRC ID |
80416
|
| 著者 |
Allen-Vercoe E, Daigneault MC, Vancuren SJ, Cochrane K, O'Neal LL, Sankaranarayanan K, Lawson PA.
|
| タイトル |
Holtiella tumoricola gen. nov. sp. nov., isolated from a human clinical sample.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
The diversity of bacteria associated with biopsy material obtained from patients with colorectal cancer was investigated using culture techniques. A novel bacterium, strain CC70AT, was isolated by diluting a sample of homogenized tissue in anaerobic medium, and then plating to yield a pure culture. Strain CC70AT was a Gram-positive, strictly anaerobic, motile, rod-shaped bacterium. Formate, but not acetate, was a fermentative end-product from growth in peptone-yeast extract and peptone-yeast-glucose broth. The G+C content of DNA from strain CC70AT was 34.9 mol%. 16S rRNA gene sequence analysis revealed that the isolate was part of the phylum Bacillota. The closest described relatives of strain CC70AT were Cellulosilyticum lentocellum (93.3 %) and Cellulosilyticum ruminicola (93.3 and 91.9% sequence similarity across 16S rRNA gene, respectively). According to the data obtained in this work, strain CC70AT represents a novel bacterium belonging to a new genus for which the name Holtiella tumoricola gen. nov., sp. nov. is proposed. The type strain for our described novel species is CC70AT (=DSM 27931T= JCM 30568T).
|
| 巻・号 |
73(7)
|
| 公開日 |
2023-7-1
|
| DOI |
10.1099/ijsem.0.005958
|
| PMID |
37436807
|
| MeSH |
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids* / chemistry
Gram-Positive Bacteria
Humans
Peptones*
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| リソース情報 |
| 一般微生物 |
JCM 30568 |
