論文 - 詳細
| RRC ID | 80510 |
|---|---|
| 著者 | Singh RP, Niharika J, Thakur R, Wagstaff BA, Kumar G, Kurata R, Patel D, Levy CW, Miyazaki T, Field RA. |
| タイトル | Utilization of dietary mixed-linkage β-glucans by the Firmicute Blautia producta. |
| ジャーナル | J Biol Chem |
| Abstract |
The β-glucans are structurally varied, naturally occurring components of the cell walls, and storage materials of a variety of plant and microbial species. In the human diet, mixed-linkage glucans [MLG - β-(1,3/4)-glucans] influence the gut microbiome and the host immune system. Although consumed daily, the molecular mechanism by which human gut Gram-positive bacteria utilize MLG largely remains unknown. In this study, we used Blautia producta ATCC 27340 as a model organism to develop an understanding of MLG utilization. B. producta encodes a gene locus comprising a multi-modular cell-anchored endo-glucanase (BpGH16MLG), an ABC transporter, and a glycoside phosphorylase (BpGH94MLG) for utilizing MLG, as evidenced by the upregulation of expression of the enzyme- and solute binding protein (SBP)-encoding genes in this cluster when the organism is grown on MLG. We determined that recombinant BpGH16MLG cleaved various types of β-glucan, generating oligosaccharides suitable for cellular uptake by B. producta. Cytoplasmic digestion of these oligosaccharides is then performed by recombinant BpGH94MLG and β-glucosidases (BpGH3-AR8MLG and BpGH3-X62MLG). Using targeted deletion, we demonstrated BpSBPMLG is essential for B. producta growth on barley β-glucan. Furthermore, we revealed that beneficial bacteria, such as Roseburia faecis JCM 17581T, Bifidobacterium pseudocatenulatum JCM 1200T, Bifidobacterium adolescentis JCM 1275T, and Bifidobacterium bifidum JCM 1254, can also utilize oligosaccharides resulting from the action of BpGH16MLG. Disentangling the β-glucan utilizing the capability of B. producta provides a rational basis on which to consider the probiotic potential of this class of organism. |
| 巻・号 | 299(6) |
| ページ | 104806 |
| 公開日 | 2023-6-1 |
| DOI | 10.1016/j.jbc.2023.104806 |
| PII | S0021-9258(23)01834-3 |
| PMID | 37172725 |
| PMC | PMC10318527 |
| MeSH | Bifidobacterium / metabolism Clostridiales* / enzymology Clostridiales* / metabolism Diet* Dietary Carbohydrates* / metabolism Gastrointestinal Microbiome* Hordeum / chemistry Humans Oligosaccharides / metabolism Probiotics beta-Glucans* / chemistry beta-Glucans* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 17581 JCM 1395 JCM 13470 JCM 1200 JCM 1275 JCM 1254 |