論文 - 詳細
| RRC ID | 80514 |
|---|---|
| 著者 | Cha GY, Seo H, Oh J, Kim BJ, Kim BJ. |
| タイトル | Potential Use of Mycobacterium paragordonae for Antimycobacterial Drug Screening Systems. |
| ジャーナル | J Microbiol |
| Abstract |
Our recent genome-based study indicated that Mycobacterium paragordonae (Mpg) has evolved to become more adapted to an intracellular lifestyle within free-living environmental amoeba and its enhanced intracellular survival within Acanthamoeba castellanii was also proved. Here, we sought to investigate potential use of Mpg for antimycobacterial drug screening systems. Our data showed that Mpg is more susceptible to various antibiotics compared to the close species M. marinum (Mmar) and M. gordonae, further supporting its intracellular lifestyle in environments, which would explain its protection from environmental insults. In addition, we developed two bacterial whole-cell-based drug screening systems using a recombinant Mpg stain harboring a luciferase reporter vector (rMpg-LuxG13): one for direct application to rMpg-LuxG13 and the other for drug screening via the interaction of rMpg-LuxG13 with A. castellanii. Direct application to rMpg-LuxG13 showed lower inhibitory concentration 50 (IC50) values of rifampin, isoniazid, clarithromycin, and ciprofloxacin against Mpg compared to Mmar. Application of drug screening system via the interaction of rMpg-LuxG13 with A. castellanii also exhibited lower IC50 values for rifampin against Mpg compared to Mmar. In conclusion, our data indicate that Mpg is more susceptible to various antibiotics than other strains. In addition, our data also demonstrate the feasibility of two whole cell-based drug screening systems using rMpg-LuxG13 strain for the discovery of novel anti-mycobacterial drugs. |
| 巻・号 | 61(1) |
| ページ | 121-129 |
| 公開日 | 2023-1-1 |
| DOI | 10.1007/s12275-022-00009-1 |
| PII | 10.1007/s12275-022-00009-1 |
| PMID | 36719620 |
| MeSH | Anti-Bacterial Agents / pharmacology Drug Evaluation, Preclinical Mycobacterium* Rifampin* / pharmacology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 17638 JCM 18565 |