| RRC ID |
80538
|
| 著者 |
Lee JY, Hong H, Lee JE, Hong YJ, Hwang HW, Jin HS, Shim H, Hong YD, Park WS, Chung JO, Lee DW.
|
| タイトル |
Valorization of leftover green tea residues through conversion to bioactive peptides using probiotics-aided anaerobic digestion.
|
| ジャーナル |
Microb Biotechnol
|
| Abstract |
Bioactive peptides (BPs) are protein fragments that benefit human health. To assess whether leftover green tea residues (GTRs) can serve as a resource for new BPs, we performed in silico proteolysis of GTRs using the BIOPEP database, revealing a wide range of BPs embedded in GTRs. Comparative genomics and the percentage of conserved protein analyses enabled us to select a few probiotic strains for GTR hydrolysis. The selected probiotics digested GTRs anaerobically to yield GTR-derived peptide fractions. To examine whether green tea (GT) peptide fractions could be potential mediators of host-microbe interactions, we comprehensively screened agonistic and antagonistic activities of 168 human G protein-coupled receptors (GPCRs). NanoLC-MS/MS analysis and thin-layer chromatography allowed the identification of peptide sequences and the composition of glycan moieties in the GTRs. Remarkably, GT peptide fractions produced by Lactiplantibacillus plantarum APsulloc 331261, a strain isolated from GT, showed a potent-binding activity for P2RY6, a GPCR involved in intestinal homeostasis. Therefore, this study suggests the potential use of probiotics-aided GTR hydrolysates as postbiotic BPs, providing a biological process for recycling GTRs from agro-waste into renewable resources as health-promoting BPs.
|
| 巻・号 |
16(2)
|
| ページ |
418-431
|
| 公開日 |
2023-2-1
|
| DOI |
10.1111/1751-7915.14155
|
| PMID |
36285915
|
| PMC |
PMC9871527
|
| MeSH |
Anaerobiosis
Humans
Hydrolases / metabolism
Peptides
Probiotics* / analysis
Tandem Mass Spectrometry*
Tea
|
| リソース情報 |
| 一般微生物 |
JCM 8130 |
