論文 - 詳細
| RRC ID | 80538 |
|---|---|
| 著者 | Lee JY, Hong H, Lee JE, Hong YJ, Hwang HW, Jin HS, Shim H, Hong YD, Park WS, Chung JO, Lee DW. |
| タイトル | Valorization of leftover green tea residues through conversion to bioactive peptides using probiotics-aided anaerobic digestion. |
| ジャーナル | Microb Biotechnol |
| Abstract |
Bioactive peptides (BPs) are protein fragments that benefit human health. To assess whether leftover green tea residues (GTRs) can serve as a resource for new BPs, we performed in silico proteolysis of GTRs using the BIOPEP database, revealing a wide range of BPs embedded in GTRs. Comparative genomics and the percentage of conserved protein analyses enabled us to select a few probiotic strains for GTR hydrolysis. The selected probiotics digested GTRs anaerobically to yield GTR-derived peptide fractions. To examine whether green tea (GT) peptide fractions could be potential mediators of host-microbe interactions, we comprehensively screened agonistic and antagonistic activities of 168 human G protein-coupled receptors (GPCRs). NanoLC-MS/MS analysis and thin-layer chromatography allowed the identification of peptide sequences and the composition of glycan moieties in the GTRs. Remarkably, GT peptide fractions produced by Lactiplantibacillus plantarum APsulloc 331261, a strain isolated from GT, showed a potent-binding activity for P2RY6, a GPCR involved in intestinal homeostasis. Therefore, this study suggests the potential use of probiotics-aided GTR hydrolysates as postbiotic BPs, providing a biological process for recycling GTRs from agro-waste into renewable resources as health-promoting BPs. |
| 巻・号 | 16(2) |
| ページ | 418-431 |
| 公開日 | 2023-2-1 |
| DOI | 10.1111/1751-7915.14155 |
| PMID | 36285915 |
| PMC | PMC9871527 |
| MeSH | Anaerobiosis Humans Hydrolases / metabolism Peptides Probiotics* / analysis Tandem Mass Spectrometry* Tea |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 8130 |