RRC ID 80645
Author Kaneshima T, Ogawa M, Yamamoto T, Tsuboyama Y, Miyata Y, Kotani T, Okajima T, Michiue T.
Title Enhancement of neural crest formation by mechanical force in Xenopus development.
Journal Int J Dev Biol
Abstract In vertebrate development, ectoderm is specified into neural plate (NP), neural plate border (NPB), and epidermis. Although such patterning is thought to be achieved by molecular concentration gradients, it has been revealed, mainly by in vitro analysis, that mechanical force can regulate cell specification. During in vivo patterning, cells deform and migrate, and this applies force to surrounding tissues, shaping the embryo. However, the role of mechanical force for cell specification in vivo is largely unknown. In this study, with an aspiration assay and atomic force microscopy, we have demonstrated that tension on ectodermal cells decreases laterally from the midline in Xenopus early neurula. Ectopically applied force laterally expanded the neural crest (NC) region, a derivative of the NPB, whereas force relaxation suppressed it. Furthermore, force application activated both the FGF and Wnt pathways, which are required for NC formation during neuroectodermal patterning. Taken together, mechanical force is necessary for NC formation in order to regulate signaling pathways. Furthermore, molecular signals specify the NP and generate force on neighboring tissue, the NPB, with its closure. This force activates signals, possibly determining the appropriate width of a narrow tissue, the NC.
Volume 68(1)
Pages 25-37
Published 2024-4-2
DOI 10.1387/ijdb.230273tm
PII 230273tm
PMID 38591691
MeSH Animals Ectoderm / metabolism Gene Expression Regulation, Developmental Neural Crest* / physiology Wnt Signaling Pathway Xenopus Proteins* / metabolism Xenopus laevis / metabolism
IF 1.105
Resource
Clawed frogs / Newts Xenopus genomic database