論文 - 詳細
| RRC ID | 80695 |
|---|---|
| 著者 | Tan S, Zhang X, Kong W, Yang XL, Molnár G, Vondráková Z, Filepová R, Petrášek J, Friml J, Xue HW. |
| タイトル | The lipid code-dependent phosphoswitch PDK1-D6PK activates PIN-mediated auxin efflux in Arabidopsis. |
| ジャーナル | Nat Plants |
| Abstract |
Directional intercellular transport of the phytohormone auxin mediated by PIN-FORMED (PIN) efflux carriers has essential roles in both coordinating patterning processes and integrating multiple external cues by rapidly redirecting auxin fluxes. PIN activity is therefore regulated by multiple internal and external cues, for which the underlying molecular mechanisms are not fully elucidated. Here, we demonstrate that 3'-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE1 (PDK1), which is conserved in plants and mammals, functions as a molecular hub that perceives upstream lipid signalling and modulates downstream substrate activity through phosphorylation. Using genetic analysis, we show that the loss-of-function Arabidopsis pdk1.1 pdk1.2 mutant exhibits a plethora of abnormalities in organogenesis and growth due to defective polar auxin transport. Further cellular and biochemical analyses reveal that PDK1 phosphorylates D6 protein kinase, a well-known upstream activator of PIN proteins. We uncover a lipid-dependent phosphorylation cascade that connects membrane-composition-based cellular signalling with plant growth and patterning by regulating morphogenetic auxin fluxes. |
| 巻・号 | 6(5) |
| ページ | 556-569 |
| 公開日 | 2020-5-1 |
| DOI | 10.1038/s41477-020-0648-9 |
| PII | 10.1038/s41477-020-0648-9 |
| PMID | 32393881 |
| MeSH | 3-Phosphoinositide-Dependent Protein Kinases / physiology* Arabidopsis / metabolism* Arabidopsis Proteins / physiology* Cell Membrane / metabolism Indoleacetic Acids / metabolism* Membrane Transport Proteins / metabolism Membrane Transport Proteins / physiology* Phospholipids / metabolism Plant Growth Regulators / metabolism* |
| IF | 13.256 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 54 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 |