RRC ID 80695
Author Tan S, Zhang X, Kong W, Yang XL, Molnár G, Vondráková Z, Filepová R, Petrášek J, Friml J, Xue HW.
Title The lipid code-dependent phosphoswitch PDK1-D6PK activates PIN-mediated auxin efflux in Arabidopsis.
Journal Nat Plants
Abstract Directional intercellular transport of the phytohormone auxin mediated by PIN-FORMED (PIN) efflux carriers has essential roles in both coordinating patterning processes and integrating multiple external cues by rapidly redirecting auxin fluxes. PIN activity is therefore regulated by multiple internal and external cues, for which the underlying molecular mechanisms are not fully elucidated. Here, we demonstrate that 3'-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE1 (PDK1), which is conserved in plants and mammals, functions as a molecular hub that perceives upstream lipid signalling and modulates downstream substrate activity through phosphorylation. Using genetic analysis, we show that the loss-of-function Arabidopsis pdk1.1 pdk1.2 mutant exhibits a plethora of abnormalities in organogenesis and growth due to defective polar auxin transport. Further cellular and biochemical analyses reveal that PDK1 phosphorylates D6 protein kinase, a well-known upstream activator of PIN proteins. We uncover a lipid-dependent phosphorylation cascade that connects membrane-composition-based cellular signalling with plant growth and patterning by regulating morphogenetic auxin fluxes.
Volume 6(5)
Pages 556-569
Published 2020-5-1
DOI 10.1038/s41477-020-0648-9
PII 10.1038/s41477-020-0648-9
PMID 32393881
MeSH 3-Phosphoinositide-Dependent Protein Kinases / physiology* Arabidopsis / metabolism* Arabidopsis Proteins / physiology* Cell Membrane / metabolism Indoleacetic Acids / metabolism* Membrane Transport Proteins / metabolism Membrane Transport Proteins / physiology* Phospholipids / metabolism Plant Growth Regulators / metabolism*
IF 13.256
Resource
Arabidopsis / Cultured plant cells, genes rpc00001