RRC ID 80707
Author Kawasumi-Kita A, Lee SW, Ohtsuka D, Niimi K, Asakura Y, Kitajima K, Sakane Y, Tamura K, Ochi H, Suzuki KT, Morishita Y.
Title hoxc12/c13 as key regulators for rebooting the developmental program in Xenopus limb regeneration.
Journal Nat Commun
Abstract During organ regeneration, after the initial responses to injury, gene expression patterns similar to those in normal development are reestablished during subsequent morphogenesis phases. This supports the idea that regeneration recapitulates development and predicts the existence of genes that reboot the developmental program after the initial responses. However, such rebooting mechanisms are largely unknown. Here, we explore core rebooting factors that operate during Xenopus limb regeneration. Transcriptomic analysis of larval limb blastema reveals that hoxc12/c13 show the highest regeneration specificity in expression. Knocking out each of them through genome editing inhibits cell proliferation and expression of a group of genes that are essential for development, resulting in autopod regeneration failure, while limb development and initial blastema formation are not affected. Furthermore, the induction of hoxc12/c13 expression partially restores froglet regenerative capacity which is normally very limited compared to larval regeneration. Thus, we demonstrate the existence of genes that have a profound impact alone on rebooting of the developmental program in a regeneration-specific manner.
Volume 15(1)
Pages 3340
Published 2024-4-22
DOI 10.1038/s41467-024-47093-y
PII 10.1038/s41467-024-47093-y
PMID 38649703
PMC PMC11035627
MeSH Animals Cell Proliferation / genetics Extremities* / physiology Female Gene Editing Gene Expression Profiling Gene Expression Regulation, Developmental* Homeodomain Proteins* / genetics Homeodomain Proteins* / metabolism Larva / genetics Larva / growth & development Male Regeneration* / genetics Regeneration* / physiology Xenopus Proteins* / genetics Xenopus Proteins* / metabolism Xenopus laevis*
IF 12.121
Resource
Clawed frogs / Newts Xenopus tropicalis