| RRC ID |
80707
|
| 著者 |
Kawasumi-Kita A, Lee SW, Ohtsuka D, Niimi K, Asakura Y, Kitajima K, Sakane Y, Tamura K, Ochi H, Suzuki KT, Morishita Y.
|
| タイトル |
hoxc12/c13 as key regulators for rebooting the developmental program in Xenopus limb regeneration.
|
| ジャーナル |
Nat Commun
|
| Abstract |
During organ regeneration, after the initial responses to injury, gene expression patterns similar to those in normal development are reestablished during subsequent morphogenesis phases. This supports the idea that regeneration recapitulates development and predicts the existence of genes that reboot the developmental program after the initial responses. However, such rebooting mechanisms are largely unknown. Here, we explore core rebooting factors that operate during Xenopus limb regeneration. Transcriptomic analysis of larval limb blastema reveals that hoxc12/c13 show the highest regeneration specificity in expression. Knocking out each of them through genome editing inhibits cell proliferation and expression of a group of genes that are essential for development, resulting in autopod regeneration failure, while limb development and initial blastema formation are not affected. Furthermore, the induction of hoxc12/c13 expression partially restores froglet regenerative capacity which is normally very limited compared to larval regeneration. Thus, we demonstrate the existence of genes that have a profound impact alone on rebooting of the developmental program in a regeneration-specific manner.
|
| 巻・号 |
15(1)
|
| ページ |
3340
|
| 公開日 |
2024-4-22
|
| DOI |
10.1038/s41467-024-47093-y
|
| PII |
10.1038/s41467-024-47093-y
|
| PMID |
38649703
|
| PMC |
PMC11035627
|
| MeSH |
Animals
Cell Proliferation / genetics
Extremities* / physiology
Female
Gene Editing
Gene Expression Profiling
Gene Expression Regulation, Developmental*
Homeodomain Proteins* / genetics
Homeodomain Proteins* / metabolism
Larva / genetics
Larva / growth & development
Male
Regeneration* / genetics
Regeneration* / physiology
Xenopus Proteins* / genetics
Xenopus Proteins* / metabolism
Xenopus laevis*
|
| IF |
12.121
|
| リソース情報 |
| ツメガエル・イモリ |
Xenopus tropicalis |
