論文 - 詳細
| RRC ID | 80773 |
|---|---|
| 著者 | Engelfriet ML, Małecki JM, Forsberg AF, Falnes PØ, Ciosk R. |
| タイトル | Characterization of the biochemical activity and tumor-promoting role of the dual protein methyltransferase METL-13/METTL13 in Caenorhabditis elegans. |
| ジャーナル | PLoS One |
| Abstract |
The methyltransferase-like protein 13 (METTL13) methylates the eukaryotic elongation factor 1 alpha (eEF1A) on two locations: the N-terminal amino group and lysine 55. The absence of this methylation leads to reduced protein synthesis and cell proliferation in human cancer cells. Previous studies showed that METTL13 is dispensable in non-transformed cells, making it potentially interesting for cancer therapy. However, METTL13 has not been examined yet in whole animals. Here, we used the nematode Caenorhabditis elegans as a simple model to assess the functions of METTL13. Using methyltransferase assays and mass spectrometry, we show that the C. elegans METTL13 (METL-13) methylates eEF1A (EEF-1A) in the same way as the human protein. Crucially, the cancer-promoting role of METL-13 is also conserved and depends on the methylation of EEF-1A, like in human cells. At the same time, METL-13 appears dispensable for animal growth, development, and stress responses. This makes C. elegans a convenient whole-animal model for studying METL13-dependent carcinogenesis without the complications of interfering with essential wild-type functions. |
| 巻・号 | 18(6) |
| ページ | e0287558 |
| 公開日 | 2023-1-1 |
| DOI | 10.1371/journal.pone.0287558 |
| PII | PONE-D-23-07410 |
| PMID | 37347777 |
| PMC | PMC10286969 |
| MeSH | Animals Caenorhabditis elegans / genetics Carcinogenesis Humans Methyltransferases / genetics Neoplasms* Peptide Elongation Factor 1 / genetics Protein Methyltransferases* |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6870 tm6890 |