論文 - 詳細
| RRC ID | 80837 |
|---|---|
| 著者 | Essig YJ, Leszczyszyn OI, Almutairi N, Harrison-Smith A, Blease A, Zeitoun-Ghandour S, Webb SM, Blindauer CA, Stürzenbaum SR. |
| タイトル | Juggling cadmium detoxification and zinc homeostasis: A division of labour between the two C. elegans metallothioneins. |
| ジャーナル | Chemosphere |
| Abstract |
The chemical properties of toxic cadmium and essential zinc are very similar, and organisms require intricate mechanisms that drive selective handling of metals. Previously regarded as unspecific "metal sponges", metallothioneins (MTLs) are emerging as metal selectivity filters. By utilizing C. elegans mtl-1 and mtl-2 knockout strains, metal accumulation in single worms, single copy fluorescent-tagged transgenes, isoform specific qPCR and lifespan studies it was possible to demonstrate that the handling of cadmium and zinc by the two C. elegans metallothioneins differs fundamentally: the MTL-2 protein can handle both zinc and cadmium, but when it becomes unavailable, either via a knockout or by elevated cadmium exposure, MTL-1 takes over zinc handling, leaving MTL-2 to sequester cadmium. This division of labour is reflected in the folding behaviour of the proteins: MTL-1 folded well in presence of zinc but not cadmium, the reverse was the case for MTL-2. These differences are in part mediated by a zinc-specific mononuclear His3Cys site in the C-terminal insertion of MTL-1; its removal affected the entire C-terminal domain and may shift its metal selectivity towards zinc. Overall, we uncover how metallothionein isoform-specific responses and protein properties allow C. elegans to differentiate between toxic cadmium and essential zinc. |
| 巻・号 | 350 |
| ページ | 141021 |
| 公開日 | 2024-2-1 |
| DOI | 10.1016/j.chemosphere.2023.141021 |
| PII | S0045-6535(23)03291-5 |
| PMID | 38151062 |
| PMC | PMC11134313 |
| MeSH | Animals Cadmium* / toxicity Caenorhabditis elegans* / metabolism Metallothionein / metabolism Metals / metabolism Protein Isoforms / genetics Protein Isoforms / metabolism Zinc / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1770 tm1700 |