論文 - 詳細
| RRC ID | 80841 |
|---|---|
| 著者 | Leboutet R, Largeau C, Müller L, Prigent M, Quinet G, Rodriguez MS, Cuif MH, Hoppe T, Culetto E, Lefebvre C, Legouis R. |
| タイトル | LGG-1/GABARAP lipidation is not required for autophagy and development in Caenorhabditis elegans. |
| ジャーナル | Elife |
| Abstract |
The ubiquitin-like proteins Atg8/LC3/GABARAP are required for multiple steps of autophagy, such as initiation, cargo recognition and engulfment, vesicle closure and degradation. Most of LC3/GABARAP functions are considered dependent on their post-translational modifications and their association with the autophagosome membrane through a conjugation to a lipid, the phosphatidyl-ethanolamine. Contrarily to mammals, C. elegans possesses single homologs of LC3 and GABARAP families, named LGG-2 and LGG-1. Using site-directed mutagenesis, we inhibited the conjugation of LGG-1 to the autophagosome membrane and generated mutants that express only cytosolic forms, either the precursor or the cleaved protein. LGG-1 is an essential gene for autophagy and development in C. elegans, but we discovered that its functions could be fully achieved independently of its localization to the membrane. This study reveals an essential role for the cleaved form of LGG-1 in autophagy but also in an autophagy-independent embryonic function. Our data question the use of lipidated GABARAP/LC3 as the main marker of autophagic flux and highlight the high plasticity of autophagy. |
| 巻・号 | 12 |
| 公開日 | 2023-7-3 |
| DOI | 10.7554/eLife.85748 |
| PII | 85748 |
| PMID | 37395461 |
| PMC | PMC10338037 |
| MeSH | Animals Apoptosis Regulatory Proteins / metabolism Autophagosomes / metabolism Autophagy Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Humans Mammals / metabolism Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Phagocytosis |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3489 tm5755 |