論文 - 詳細
| RRC ID | 80852 |
|---|---|
| 著者 | Tran MV, Khuntsariya D, Fetter RD, Ferguson JW, Wang JT, Long AF, Cote LE, Wellard SR, Vázquez-Martínez N, Sallee MD, Genova M, Magiera MM, Eskinazi S, Lee JD, Peel N, Janke C, Stearns T, Shen K, Lansky Z, Magescas J, Feldman JL. |
| タイトル | MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement. |
| ジャーナル | Dev Cell |
| Abstract |
Microtubule doublets (MTDs) comprise an incomplete microtubule (B-tubule) attached to the side of a complete cylindrical microtubule. These compound microtubules are conserved in cilia across the tree of life; however, the mechanisms by which MTDs form and are maintained in vivo remain poorly understood. Here, we identify microtubule-associated protein 9 (MAP9) as an MTD-associated protein. We demonstrate that C. elegans MAPH-9, a MAP9 homolog, is present during MTD assembly and localizes exclusively to MTDs, a preference that is in part mediated by tubulin polyglutamylation. We find that loss of MAPH-9 causes ultrastructural MTD defects, including shortened and/or squashed B-tubules with reduced numbers of protofilaments, dysregulated axonemal motor velocity, and perturbed cilia function. Because we find that the mammalian ortholog MAP9 localizes to axonemes in cultured mammalian cells and mouse tissues, we propose that MAP9/MAPH-9 plays a conserved role in regulating ciliary motors and supporting the structure of axonemal MTDs. |
| 巻・号 | 59(2) |
| ページ | 199-210.e11 |
| 公開日 | 2024-1-22 |
| DOI | 10.1016/j.devcel.2023.12.001 |
| PII | S1534-5807(23)00651-2 |
| PMID | 38159567 |
| MeSH | Animals Axoneme* / metabolism Axoneme* / ultrastructure Caenorhabditis elegans* / metabolism Cilia / metabolism Mammals Mice Microtubules / metabolism Movement Tubulin / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3310 tm4059 tm3889 tm3360 tm3871 |