論文 - 詳細
| RRC ID | 80891 |
|---|---|
| 著者 | Pastore B, Hertz HL, Tang W. |
| タイトル | Pre-piRNA trimming safeguards piRNAs against erroneous targeting by RNA-dependent RNA polymerase. |
| ジャーナル | Cell Rep |
| Abstract |
The Piwi/Piwi-interacting RNA (piRNA) pathway protects genome integrity in animal germ lines. Maturation of piRNAs involves nucleolytic processing at both 5' and 3' ends. The ribonuclease PARN-1 and its orthologs mediate piRNA 3' trimming in worms, insects, and mammals. However, the significance of this evolutionarily conserved processing step is not fully understood. Employing C. elegans as a model, we recently discovered that 3' trimming protects piRNAs against non-templated nucleotide additions and degradation. Here, we find that worms lacking PARN-1 accumulate an uncharacterized RNA species termed anti-piRNAs, which are antisense to piRNAs. Anti-piRNAs associate with Piwi proteins, are 17-19 nucleotides long, and begin with 5' guanine or adenine. Untrimmed pre-piRNAs are misdirected by the terminal nucleotidyltransferase RDE-3 and RNA-dependent RNA polymerase EGO-1, leading to the formation of anti-piRNAs. This work identifies a class of small RNAs in parn-1 mutants and provides insight into the activities of RDE-3, EGO-1, and Piwi proteins. |
| 巻・号 | 43(2) |
| ページ | 113692 |
| 公開日 | 2024-2-27 |
| DOI | 10.1016/j.celrep.2024.113692 |
| PII | S2211-1247(24)00020-2 |
| PMID | 38244197 |
| PMC | PMC10949418 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins* / genetics Mammals Nucleotides Nucleotidyltransferases Piwi-Interacting RNA RNA RNA-Dependent RNA Polymerase* Ribonucleases |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm869 tm872 |